Method for culturing avian primordial germ cells (PGCs) for a long period and preparing a medium for culturing avian PGCs for a long period

摘要

The present invention is a method for culturing avian PGCs comprising providing fibroblast cells, culturing fibroblast cells in a basal medium, collecting a conditioned medium, providing avain gonadal cells with PGCs from sex glands, and growing the PGCs in the conditioned medium supplemented with growth factors, wherein the conditioned medium provides the avian PGCs being culturing for a long period. Furthermore, the present invention is a method for preparing a medium for culturing avian PGCs comprising providing a basal medium, culturing fibroblast cells in the basal medium, collecting a conditioned medium; and adding growth factors into the conditioned medium, wherein the conditioned medium provides the avian PGCs being cultured for a long period. The present invention is the conditioned medium prepared by the above method.