Effect of mitomycin combined with Nd-YAG laser on cell proliferation and invasion as well as MEK/ERK signaling pathway in obstructive lacrimal duct model

摘要

Objective: To study the effect of mitomycin (MMC) combined with Nd-YAG laser on cellproliferation and invasion as well as MEK/ERK signaling pathway in obstructive lacrimal ductmodel. Methods: New Zealand rabbits were selected as experimental animals and dividedinto model group, laser group and MMC + laser group; obstructive lacrimal duct model wasestablished, then laser group were given Nd-YAG laser intervention, and MMC + laser groupwere given Nd-YAG laser combined with mitomycin intervention. 2 months after intervention,the expression of proliferation molecules, invasion molecules and MEK-ERK signalingmolecules in lacrimal duct tissue were measured. Results: TGF-β, CTGF, PCNA, Ki-67,Col-I, Col-III, MEK, ERK1/2, MMP2 and MMP9 protein levels in lacrimal duct tissue of lasergroup were significantly higher than those of model group while TSG-6, Cthrc1 and TIMP1protein levels were significantly lower than those of model group; TGF-β, CTGF, PCNA, Ki-67, Col-I, Col-III, MEK, ERK1/2, MMP2 and MMP9 protein levels in lacrimal duct tissue ofMMC + laser group were significantly lower than those of laser group while TSG-6, Cthrc1and TIMP1 protein levels were significantly higher than those of laser group. Conclusion:Mitomycin can inhibit cell proliferation and invasion as well as MEK/ERK signaling pathwayactivation in obstructive lacrimal duct model after Nd-YAG laser treatment.