The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P <0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.
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机译：在用黑曲霉发酵大豆废料的过程中，确定纤维素酶的活性和植物雌激素（染料木黄酮）的形成含量。在0、48、96、144和192小时的发酵孵育时间以及添加微量营养素（V2）和不添加微量营养素（V1）的条件下进行分析。使用T检验对在植物雌激素形成材料上的纤维素酶活性的数据进行分析。发酵大豆废料中纤维素酶的平均含量在48小时后从0.032-0.139 IU / ml（V1）和0.061-0.158 IU / ml（V2）增加到0.238 IU / ml（V1）和0.245 IU / ml（V2） 192小时发酵大豆废料中的染料木素平均水平在48小时后也从0.101-0.573 mg / g（V1）和0.114-0.587 mg / g（V2）增加，并增加到0.722 mg / g（V1）和0.699 192小时后为mg / g（V2）。统计分析表明，处理V1和V2对增加纤维素酶含量和染料木黄酮含量有影响（P <0.05）。黑曲霉发酵大豆废料中纤维素酶和染料木素的酶活性之间的相关性很显着（R2 = 0,949）。酶性能与其形成材料之间的关系是协同运行的，在发酵过程中纤维素酶活性和染料木黄酮水平显着增加。
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