Determination of Triacylglycerol Composition of Trichilia emetica Seed Oil Using GC-MS and ¹H-NMR Spectroscopy

摘要

Trichilia emetica seed oil was analysed for fatty acid profiles using GC-MS and proton nuclear magnetic resonance spectroscopy (¹H-NMR). The percentage triacylglycerol conversion to its corresponding methyl esters was determined by ¹H NMR and was calculated to be 94%. A total of six FAMEs were identified in the seed oil by the NIST library match software and the fragmentation pattern data of GC/MS. The results obtained from the two methods were 55% saturated (C14:0, C16:0, C18:0), 35% monounsaturated (C18:1) and 10 % polyunsaturated (C18:2 and C18:3). Generally, the ¹H-NMR results were in good agreement with gas chromatographic (GC) analyses. In conclusion, the ¹H-NMR-based procedures is fast as it does not require the oil to be derivatized and can be applied to neat T. emetica seed oil.