Association of preoperative EpCAM Circulating Tumor Cells and peripheral Treg cell levels with early recurrence of hepatocellular carcinoma following radical hepatic resection

摘要

Background This study was carried out to determine the prognostic significance of preoperative peripheral epithelial cell adhesion molecule- positive ( EpCAM +) circulating tumor cell (CTC) and T regulatory (Treg) cell levels in hepatocellular carcinoma (HCC) patients for the prediction of postoperative recurrence following curative resection. Methods A total of 49 patients about to undergo curative resection for HCC were recruited into the study. PCR and FACS were used to detect the preoperative levels of EpCAM mRNA+ CTCs and CD4+CD25+Foxp3+ Treg cells. The prognostic value of EpCAM mRNA+ CTCs, CD4+CD25+Foxp3+ Treg cells, and other clinicopathological factors were analyzed by applying the Kaplan–Meier method and the multivariate Cox proportional hazards model. Results The number of EpCAM mRNA+ CTCs and Treg/CD4+ cells showed significant correlation as prognostic factors of postoperative HCC recurrence: EpCAM mRNA+ CTC?≥?2.22 (P?=?0.001) and Treg/CD4+?≥?5.07 ( P =?0.045), with EpCAM mRNA+ CTC?≥?2.22 ( P =?0.003, HR?=?6.668) being the most important indicator. Patients with high CTC/Treg levels showed a significantly higher risk of developing postoperative HCC recurrence than those with low CTC/Treg levels (66.7?% vs. 10.3?%, P Conclusions Elevated EpCAM mRNA+ CTC and Treg/CD4+ levels were associated with early recurrence of HCC, indicative of poor clinical outcome. The combined detection of EpCAM mRNA+ CTC and Treg/CD4+ may therefore provide a novel prognostic predictor for HCC patients.