Assessing the ability of Lactobacillus rhamnosus GG to bind aflatoxin B1 from contaminated cottonseed

摘要

Biological decontamination of mycotoxins using microorganisms is one of the well known strategies for the management of mycotoxins in foods and feeds. In this study, the interaction of aflatoxin B1 (AFB1) in cottonseed with Lactobacillus rhamnosus strain GG was investigated for the first time. AFB1 at concentrations (5, 10 and 20 μg/l) was added to the cottonseed meal in buffer phosphate solution and then bacterial culture (109 CFU/ml) in MRS broth medium was added to the solution and incubated at 25???°C for 4, 12 and 24 hrs. The aflatoxin binding capacity of the strain was quantified by the amount of unbound AFB1 using ELISA technique. Results showed the binding capacity of viable, heat killed and acid killed bacteria respectively 44, 47 and 49%. Removal ofAFB1 by this strain was a slow process with approximately 41%AFB1 removal at both 12 and also 24 hrs. The primary concentration ofAFB1 did not influence the efficacy of detoxification (p>0.05). These findings further support the ability of specific strains of lactic acid bacteria to bind feed contaminants.