Expression of Chlamydomonas actin-gfp fusion gene in to - bacco suspension cell and polymerization of the actin - gfp protein in vitro

摘要

The fusion gene of actin (cDNA of Chlamydo- monas reinhardtii) and green fluorescence protein (gfp) had been constructed into two expression vectors which could be expressed in E. coli and tobacco suspension cells BY2. The corrrect expression was observed in E. coli and BY2. The fluorescence microscopy.