DNA Mismatch Repair Deficiency Detection in Colorectal Cancer by a New Microsatellite Instability Analysis System

摘要

MSI and IHC results for case 50N/T. Panels A-D were MSI test electropherograms using ProDx® MSI kit. Panels E-F were MSI test electropherograms using MSI1.2 kit. Panel H was MSI test electropherogram with three dinucleotide repeat markers from NCI panel. For each panel, top trace was from normal tissue, and bottom trace was from the tumor tissue. Red arrows indicated unstable markers. Bottom panels were immunohistochemistry staining for 4 MMR proteins in tumor tissue with anti-MLH1, MSH2, MSH6, and PMS2 antibodies, respectively