Long non-coding RNA MIR100HG promotes the migration invasion and proliferation of triple-negative breast cancer cells by targeting the miR-5590-3p/OTX1 axis

摘要

MIR100HG and miR-5590-3p expression patterns in TNBC cells and tissues and demonstration that MIR100HG directly targets miR-5590-3p. a The relative expression of MIR100HG and miR-5590-3p in TNBC tissues and their paired non-cancerous tissues (N = 20), as detected by RT-qPCR. b The expression level of MIR100HG and miR-5590-3p expression in TNBC and human breast epithelial cell lines, as detected by RT-qPCR. c The predicted miR-5590-3p binding site on MIR100HG. d MiR-5590-3p expression in TNBC cells after being transfected with the miR-5590-3p mimics and inhibitor. e The luciferase activity of MIR100HG-WT and MIR100HG-MUT in HEK-293T cells transfected with the miR-5590-3p mimics and inhibitor. f Relative expression level of MIR100HG and miR-5590-3p following Ago2 immunoprecipitation or IgG control immunoprecipitation.*P < 0.05, **P < 0.01, ***P < 0.001