Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells

摘要

Schematic flow of our platform. Types of introducible mutations. Thirteen of the canonical amino acids can be converted to another amino acids. Fraction of each introducible type of mutation. Targeted cytosine to thymine substitution efficiency in the “activity window” of the base editor. Solid line represents the median, with the upper and lower limits of each box corresponding to the 75 and 25% quantiles of the data. All p-values determined by two-sided Student’s -test, **  n = 4 independent base positions). Allele frequency of the E203K mutation before and 25 days after drug treatment. Efficiency of cytosine substitution according to position in the protospacer of the sgRNA.