Coexpression of CD163 and CD141 identifies human circulating IL-10-producing dendritic cells (DC-10)

摘要

Transcriptional profile of in vitro differentiated DC-10. Mature (m)DCs, immature (i)DCs, DC-10, and DC-10 activated with LPS (DC-10+LPS) were differentiated in vitro from peripheral blood monocytes of healthy donors (  = 4) according to the Methods section, and microarray analysis was performed. Two-dimensional heatmap of significant differentially expressed genes (DEGs) (  1). The color scale is relative to the mean expression levels. Genes are in columns, and samples are in rows. Data in rows have been hierarchically clustered. Scatterplots of differential expression between mDCs, iDCs, DC-10, and DC-10+LPS. Each dot represents the averaged Log FC value of a single gene. Dashed diagonal lines indicate the thresholds for FC (absolute FC value >1). Numbers indicate DEGs. Principal component analysis plot for expression data in mDCs, iDCs, DC-10, and DC-10+LPS