CD8-positive memory T cells in tumor-draining lymph nodes of patients with breast cancer

摘要

Phenotype determination of CD8 memory T cell subsets in tumor-draining lymph nodes of patients with breast cancer. After selecting CD8 positive cells in the lymphocyte gate ( ), the phenotype of different subsets was defined based on the expression of CCR7, CD45RO and CD95. CD95 expressing CCR7 CD45RO CD8 lymphocytes were considered as TCM cells in both CD45RO and CD45RO populations ( and ), whereas lymphocytes with a CCR7 CD45RO CD95 CD8 phenotype were considered as TEM cells ( ) and CCR7 CD45RO cells that did not express CD95 were considered as naive cells ( ). A subgroup of lymphocytes with naive phenotype (CCR7 CD45RO ) but positive for CD95 was coined as TSCM cells ( ). TSCM: T memory stem; TCM: T central memory; TEM: T effector memory cells; TN: T naive