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Estimation of Minced Pork Microbiological Spoilage through Fourier Transform Infrared and Visible Spectroscopy and Multispectral Vision Technology

机译:傅里叶变换红外可见光谱和多光谱视觉技术估算猪肉碎肉的微生物变质

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摘要

Spectroscopic and imaging methods coupled with multivariate data analysis have been increasingly studied for the assessment of food quality. The objective of this work was the estimation of microbiological quality of minced pork using non-invasive spectroscopy-based sensors. For this purpose, minced pork patties were stored aerobically at different isothermal (4, 8, and 12 °C) and dynamic temperature conditions, and at regular time intervals duplicate samples were subjected to (i) microbiological analyses, (ii) Fourier transform infrared (FTIR) and visible (VIS) spectroscopy measurements, and (iii) multispectral image (MSI) acquisition. Partial-least squares regression models were trained and externally validated using the microbiological/spectral data collected at the isothermal and dynamic temperature storage conditions, respectively. The root mean squared error (RMSE, log CFU/g) for the prediction of the test (external validation) dataset for the FTIR, MSI, and VIS models was 0.915, 1.173, and 1.034, respectively, while the corresponding values of the coefficient of determination (R2) were 0.834, 0.727, and 0.788. Overall, all three tested sensors exhibited a considerable potential for the prediction of the microbiological quality of minced pork.
机译:越来越多地研究了光谱和成像方法以及多变量数据分析来评估食品质量。这项工作的目的是使用基于非侵入式光谱的传感器评估猪肉末的微生物质量。为此,将切碎的肉饼在不同的等温(4、8和12°C)和动态温度条件下需氧存储,并以固定的时间间隔对重复的样品进行(i)微生物分析,(ii)傅里叶变换红外光谱(FTIR)和可见(VIS)光谱测量,以及(iii)多光谱图像(MSI)采集。分别训练了偏最小二乘回归模型并使用在等温和动态温度存储条件下收集的微生物/光谱数据进行了外部验证。预测FTIR,MSI和VIS模型的测试(外部验证)数据集的均方根误差(RMSE,log CFU / g)分别为0.915、1.173和1.034,而系数的相应值测定值(R 2 )为0.834、0.727和0.788。总体而言,所有这三个经过测试的传感器都具有预测猪肉碎微生物质量的巨大潜力。

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