Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum. The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g. Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method. The DDFPs50 exhibited the highest antioxidant activity compared to the other fractions. The molecular weight, polydispersity, and conformation of these fractions were also characterized. In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant. This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.
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机译：采用响应面法（RSM）优化了从石dev石flowers花中超声波辅助提取（UAE）多糖的条件。 DDFPs的最大产量的最佳条件如下：提取温度为63.13°C，提取时间为53.10 min，水与原料的比为22.11 mL / g。此外，通过逐步乙醇沉淀法从石end石flowers花多糖（DDFPs）制备了三个馏分（DDFPs30，DDFPs50和DDFPs70）。与其他馏分相比，DDFPs50具有最高的抗氧化活性。这些馏分的分子量，多分散性和构象也得到了表征。特别地，对DDFP的单糖组成分析表明，甘露糖和葡萄糖是主要成分，类似于D.officinale植物的那些。这项研究为DDFP的生产提供了一种快速提取技术和必要的信息，这些DDFP可能被用作保健食品。
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