Taking Onchidium struma as the raw material, its polysaccharides was extracted by the traditional method of hot water. Process of extracting polysaccharides fromO. struma was optimized by response surface methodology. Through the single factor experiment, the effects of extraction temperature, extraction time, and liquor-to-material ratio to the extraction rate of polysaccharide were investigated. Furthermore, the experiments were done by Box-Behnken, and the data was analyzed by Design-Expert 8.05 software. Antioxidant activity ofO. strumapolysaccharides was determined by the capacity of scavenging free hydroxyl radical and DPPH radical. On the basis of the analysis of regression equation, determination of factors affecting the extraction of polysaccharide was conducted by the extraction rate of polysaccharide served as response value. Extraction temperature at 92℃, extraction time of 30 h, and liquor-to-material ratio of 1∶29 g/mL, leaching rate ofO. strumapolysaccharide reached 9.206%.O. struma polysaccharide had ability to clear off free hydroxyl radical and DPPH radical. Conclusively, the extraction technology is reasonable and reliable for the extraction of polysaccharidesO. struma, which provides the theoretical support for the future industrial production, and itspolysaccharides have significant antioxidation in vitro.%采用传统的热水浸提法提取瘤背石磺多糖，运用响应面法优化多糖的提取工艺，并以粗多糖对其体外抗氧化活性作初步研究。通过单因素实验，探讨浸提温度、浸提时间、料液比对瘤背石磺多糖提取率的影响并选取实验水平，采用 Box-Behnken设计实验方案，利用Design-Expert 8.05软件分析数据。通过清除羟基自由基和 DPPH自由基的能力来检测多糖抗氧化性能。结果显示，最佳水提条件为浸提温度92℃、浸提时间30 h、料液比1∶29（g/mL），在最优工艺条件下瘤背石磺多糖的提取率为9.206%，瘤背石磺多糖对羟基自由基和DPPH自由基都具有一定的清除率。采用该方法优化提取多糖，合理可靠，为以后的工业化生产提供理论依据，且多糖具有明显的体外抗氧化活性。
展开▼
