水酶法提取板栗壳棕色素工艺条件的筛选

摘要

为了确定水酶法提取板栗壳棕色素的工艺条件,以板栗壳为原料,通过单因素试验和正交试验,对其工艺条件进行筛选.结果表明,水酶法提取板栗壳棕色素的最适工艺条件为:复合酶总酶量2.0%,纤维素酶、中性蛋白酶、果胶酶质量比为2:3:5,酶处理pH值5.0,温度50℃,时间2.0 h,料液比1:120.%In order to obtain the optimal extraction process conditions of brown pigmentfrom chestnut shell by enzymatic hydrolysismethod, taking chestnut shellasmaterials, the process conditionswere screened by several single factor experimentsanda orthogonal experiment. Theresults showed that the optimal extraction process conditionswere 2.0% composite enzymes, themassratio 2:3:5 of cellulose, neutral proteaseand pectinase,reaction pHvalue 5.0, temperature 50℃, duration 2.0 h,and solid-liquidratio 1:120.