3种金属烤瓷冠修复后龈沟液中白细胞介素-8水平动态测定

摘要

目的 探讨镍铬合金、钴铬合金、金合金3组金属烤瓷冠修复前后不同时期龈沟液(GCF)内白细胞介素-8(IL-8)水平变化,了解这3种金属烤瓷冠在不同时期对牙龈的刺激程度.方法 临床随机选择3种金属烤瓷冠修复患者共45例,每组各15例,测定在修复前以及修复后1、3、6个月GCF量,并采用双抗体夹心酶联免疫吸附法检测同期GCF中IL-8总量、IL-8含量.结果 镍铬合金烤瓷冠在修复1、3、6个月后GCF量、IL-8总量与修复前相比差异均有统计学意义(P＜0.05).钴铬合金、金合金烤瓷冠在修复3个月后回复到修复前水平.3种金属烤瓷冠IL-8含量在不同时期差异均无统计学意义.结论 IL-8参与炎症的免疫调节,可作为评价不同金属烤瓷冠对牙龈组织刺激程度的指标,不同合金烤瓷冠内冠材料对牙龈存在不同的长期刺激,临床金属烤瓷冠修复时对内冠材料的选择应予以关注.%Objective To study the interleukin-8 (IL-8) levels in gingival crevicular fluid (GCF) of porcelain teeth coated with Ni-Cr, Co-Cr or gold alloy at different time periods, and to uncover the degree of stimulation by these alloys on gingiva at different time periods. Methods 45 cases of porcelain teeth coated with Ni-Cr, Co-Cr or gold alloy were selected randomly, with 15 cases in each group. Sandwich enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration and total amount of IL-8 in GCF. The assay was done before treatment, as well as at 1, 3 and 6 months post-treatment. Results The total amounts of IL-8 and GCF volume in the Ni-Cr alloy coated porcelain teeth were higher in different time period than those before treatment (P<0.05). However, the IL-8 levels in Co-Cr and gold alloy coated porcelain teeth returned to pre-dental restoration after 3 months. Otherwise, the levels of IL-8 concentration in GCF showed no significant difference among the three different alloys coating at different time periods. Conclusion IL-8 is an important cytokine during tissue inflammation. Therefore, the level of IL-8 in GCF is a useful criteria for the evaluation of stimulation degree on gingiva by different alloy coating materials in porcelain teeth. The clinical choice of different alloy coating for porcelain teeth should be considered accordingly.