微流控PCR装置中温度控制技术评述

摘要

在微流控PCR装置中,温度直接影响PCR扩增结果,精确控制温度是微流控PCR的一个关键问题.温度控制包括系统加热方式和冷却方式,温度的测量和控制算法.加热和冷却有多种不同的实现方式,控制算法基本采用PID算法.总结了微流控PCR温度控制系统的加热方式、冷却方式和测量方式,并比较了各种方法的优缺点.%In PCR microfluidic devices, the result of PCR amplification is directly affected by temperature, so how to control the temperature for PCR microfluidics precisely is a crucial issue. The temperature control includes heating ways, cooling ways, measurement of temperature and control algorithm. There are many kinds of ways for heating and cooling, while the most commonly used temperature control algorithm is accomplished through a proportional/integral/differential module. The heating ways, cooling ways and temperature measurement for PCR microfluidics are summarized and compared in terms of advantages and disadvantages.