PCR chip has widely applications in many aspect,especial y in biological engineering and medicine.Therefore based on MEMS technolongy researchers designed and fabricated a PCR chip with sensing eletrode by temperature control ing.This whole system has two parts,including the structure of PCR-CE chip,designing of program and circiut with temperture control system.This paper describes composition of PCR-CE chip,fabrication process,temperature control program design,circuit control ing and application.At last,the operability of microfliudic chip was verified through utilazing the related experiments.% 微流控芯片在生物工程、医学等领域有很广泛的应用背景。基于MEME工艺技术研究人员设计并制作一种可以实时温度控制的PCR-CE微流控芯片。其中芯片系统设计主要包括芯片结构的制作和温度控制电路的设计两部分,本文详细介绍了芯片的组成、制作过程、温度控制电路的硬件系统设计、程序设计等主要内容,并进行相关实验验证了芯片的可操作性。
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