清热合剂对上呼吸道感染常见致病菌的体外抑菌试验

摘要

Objective To observe the antimicrobial effect of a kind of Chinese medicine Qingre compound preparation on the common pathogenic bacteria of upper respiratory tract infection (URTI). Methods A total of 163 common pathogen⁃ic bacteria of URTI was selected in this study, including 74 non extended-spectrum β- lactamases (ESBLs)-producing Gram-negative bacteria (33 Escherichia coli, 24 Klebsiella pneumonia and 17 Pseudomonas aeruginosa), 10 ESBLs-produc⁃ing Gram-negative bacteria (6 Escherichia coli and 4 Klebsiella pneumoniae) and 79 Gram-positive bacteria [11 methicil⁃lin-resistant Staphylococcus aureus (MRSA), 46 methicillin-sensitive Staphylococcus aureus and 22 Streptococcus pneu⁃moniae]. Agar dilution method was adopted to perform the quantitative drug sensibility test. Agar plates that contained differ⁃ent concentrations of Qingre compound preparation were prepared. The bacterial suspension was planted on the plates. Then we observed the plates after incubation, and recorded the minimum inhibitory concentration (MIC). Results The antimicro⁃bial rates of Qingre compound preparation were 88, 176 and 22 g/L for MIC90 of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The antimicrobial effects of Qingre compound preparation were coincident on the MIC 90 of ES⁃BLs-producing strains and non ESBLs-producing strains. The accumulated antibacterial rates of different concentrations of medicine to Pseudomonas aeruginosa were the highest. The MIC90 values of Qingre compound preparation were 11, 11 and 22 g/L for MSSA, MRSA and Streptococcus pneumoniae. The MIC90 of MRSA was coincident with MSSA, but MIC50 of MRSA was slightly higher than that of MSSA. The accumulated antibacterial rates of different concentrations of medi cine to MSSA and MRSA were all higher than those of Streptococcus pneumonia. The accumulated antibacterial rate of MSSA was similar with that of MRSA. Conclusion The Chinese medicine Qingre compound preparation could restrain common patho⁃genic bacteria of URTI except Klebsiella pneumoniae. The antibacterial effect of Qingre compound preparation is significant⁃ly better in Ggram-positive bacteria than that of Gram-negative bacteria.%目的：观察清热合剂对上呼吸道感染常见致病菌的体外抑菌活性。方法上呼吸道感染常见致病菌163株中不产超广谱β-内酰胺酶（ESBLs）革兰阴性菌74株（大肠埃希菌33株，肺炎克雷伯菌24株，铜绿假单胞菌17株）；产ESBLs革兰阴性菌10株（大肠埃希菌6株，肺炎克雷伯菌4株）；革兰阳性菌79株[耐甲氧西林金黄色葡萄球菌（MRSA）11株、甲氧西林敏感金黄色葡萄球菌（MSSA）46株，肺炎链球菌22株]。采用琼脂稀释法对清热合剂进行定量抑菌试验，配制含有不同药物浓度的琼脂平板，在平板上接种待测菌株菌悬液，孵育后观察含药平板，记录最低抑菌浓度（MIC）。结果清热合剂对大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌等革兰阴性菌的MIC90分别为88、176、22 g/L，其对产ESBLs与不产ESBLs革兰阴性菌的抑菌效果一致；不同浓度药物对铜绿假单胞菌的累积抑菌率均最高。清热合剂对MSSA、MRSA和肺炎链球菌等革兰阳性菌的MIC90分别为11、11、22 g/L，MRSA的MIC90与MSSA相同，但MIC50略高于MSSA；不同浓度药物对MSSA和MRSA的累积抑菌率均高于肺炎链球菌，对MSSA与MRSA的累积抑菌率相近。结论清热合剂对上呼吸道感染常见的致病菌除肺炎克雷伯菌之外均有一定的抑菌作用，对革兰阳性菌的抑菌效果明显优于革兰阴性菌。