咔唑比色法测定掺假蜂蜜中果胶的含量

摘要

A method for determination of pectin content in honey was established by carbazole colorimetry. The pectin was extracted by 63 ％ hot ethonal to remove the sugar in honey. The results showed that the top grade concentrated sulphurie acid should be used during the experience. And the ethonal, which was used to dilute carbazole, should be pured. The recovery of pectin was 88.2％ ～ 94.6％ with the detectable limit of 0.15 mg/kg.%建立了咔唑比色法测定蜂蜜中掺假果胶含量的方法.采用63%热乙醇溶液洗除蜂蜜中的糖分,提取试样中的果胶.结果表明,试验过程中试剂要采用优级纯浓硫酸,配制咔唑显色剂的乙醇使用前要进行精制.半乳糖醛酸的加标回收率为88.2%-94.6%,检出限为0.15 mg/kg.