RNF8在不同肿瘤细胞中的表达以及与细胞辐射敏感性相关性研究

摘要

Objective : To determine the RNF8 expression in different tumor cell lines and assess the relationship between RNF8 and the tumor cell radiation sensitivity.Method :The RNF8 protein expression was determined by western blot in U87、EC109、Hela、SGC7901 cell lines.the tumor cell radiation sensitivity was analyzed by MTT assay.Results : The RNF8 expression was different in different tumor cells.Different radiation sensitivity was observed in 4 tumor cells.Conclusion:There is a positive correlation between the expressions of RNF8 and the radiation sensitivity of the tumer cells , which may be a potential index for radiation sensitivity.%目的:测定不同肿瘤细胞中RNF8蛋白的表达以及与细胞辐射敏感性的关系.方法:采用Western blot 方法测定U87、EC109、Hela、SGC7901等肿瘤细胞中RNF8蛋白的表达量;采用MTT法测定各个肿瘤细胞的辐射敏感性.结果:RNF8在不同的肿瘤细胞中表达量不同,不同肿瘤细胞具有不同辐射敏感性.结论:RNF8与肿瘤细胞辐射敏感性正相关,有可能成为辐射敏感检测指标.