体外化学模拟体系中苹果多酚抗氧化及清除亚硝酸根离子活性的研究

摘要

通过测定苹果多酚对羟自由基、超氧阴离子自由基、DPPH自由基、亚硝酸根离子的清除作用,对脂质过氧化和β-胡萝卜素/亚油酸自氧化体系的抑制作用,以及还原能力等实验,研究了苹果多酚的体外抗氧化活性,并与VC进行了对比。实验结果表明,苹果多酚对羟自由基、超氧阴离子自由基、DPPH自由基、亚硝酸根离子均有不同程度的清除作用,对脂质过氧化和β-胡萝卜素/亚油酸自氧化体系有一定的抑制作用,对Fe3＋具有良好的还原能力。因此,苹果多酚是一种良好的天然抗氧化及清除亚硝酸根离子的活性物质。%The antioxidant effect of apple polyphenols from apple pomace was studied by evaluations of hydroxy radical scavenging activity,superoxide radical scavenging activity,DPPH radical scavenging activity,nitrite scavenging activity,lipid oxidation inhibiting activity,β-carotin/linolic acid oxidation inhibiting,and reducing power.Results showed that apple polyphenols had significant hydroxy radical scavenging activity,superoxide radical scavenging activity,DPPH radical scavenging activity and nitrite scavenging activity,and also significant in reducing power,lipid oxidation inhibiting activity and β-carotin/linolic acid oxidation inhibiting activity.It was indicated that apple polyphenols from apple pomace had high capacity of antioxidation and nitrite scavenging activity.