鱼鳞胶原蛋白提取过程中的脱钙工艺条件优化

摘要

Optimization of decalcification process during extracting collagen from scale of sea fish was investigated.Orthogonal experiment method was applied to estimate the effect of acid concentration, temperature and time on the decalcification of fish scale. Ion chromatography was applied to observe the effect of factors on the dissolution of collagen from scale while decalcifying. Optimized process of collagen extraction from sea fish scale was confirmed. Temperature was 18℃,acid concentration was 0.7mol/L and the time was 24h. The process was carried out at large scale,the result indicated it was perfect for plant scale with decalcification rate at 99.4％.%研究了海鱼鱼鳞胶原蛋白提取过程中的脱钙工艺条件优化.采用L9(34)正交实验,考察了酸浓度、温度、时间等因素对鱼鳞脱钙工艺的影响,并使用离子色谱法考察了各种工艺条件对鱼鳞胶原蛋白在脱钙过程中溶出的影响.确定了鱼鳞胶原蛋白提取过程中的脱钙优化工艺,即温度18℃,酸浓度0.7mol/L,时间24h.放大实验至原料2kg规模,得脱钙率为99.4%,适合规模化生产.