The scales is a kind of precious resource with high nutritional value. The collagen protein from scales have a widely usage prospection in the areas of health food, cosmetics and so on, and they are worthy of further studied. In this paper, the method of extracting collagen protein from the scales of Tilapia was studied by pepsin hydrolysis. First, the calcium was demineralized from the scales with using of 0.5 mol/L EDTA solution, then, the scales hydrolyzed by pepsin was made to extract the collagen, and the content of L - hydroxyproline was taken as an index to measure the extraction percentage. On this basis, the collagen extract conditions was discussed from the Tilapia scales by the pepsin. It was showed that the pepsin hydrolysis conditions were the enzyme dosage 3%, citric acid 12 g/L, hydrolysis time 20h, so the rate of collagen extraction is higher.
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机译：鳞片是一种具有高营养价值的珍贵资源。来自鳞片的胶原蛋白蛋白质在健康食品，化妆品等领域具有广泛使用的借鉴，并且值得进一步研究。本文研究了从百草林水解中研究了来自罗非鱼鳞片的胶原蛋白蛋白质的方法。首先，通过使用0.5mol / L EDTA溶液，钙从鳞片中脱矿质，然后，通过胃蛋白酶水解的鳞片提取胶原蛋白，并将L - 羟基脯氨酸的含量作为测量提取百分比的指数。在此基础上，从百草林中讨论了胶原蛋白的提取物条件。结果表明，胃蛋白酶水解条件是酶剂量3％，柠檬酸12g / L，水解时间20h，因此胶原萃取率较高。
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