库拉索芦荟凝集素的分离与纯化

摘要

Objective: To isolate and purify lectin from the aloe barbadensis miller. Methods: Pounded by homogenate,salting-out and dialysis,the extract lectin of aloe from aloe barbadensis miller was obtained. Rabbit blood cells were used to check the aloe crude extract activity. The protein fractions were determined by SDSpolyacrylamide gel electrophoresis and filtration chromatography of glucan G-75 gel. Coagulation activity test was also used to prove the fractions. Elution from the liquid was enriched. The characteristic of the fraction was examined by SDS-polyacrylamide gel electrophoresis and filtration chromatography of glucan G-200 gel. Results: Results showed the extract could agglutinate the rabbit erythrocytes. The lectin proved to be existed in the crude extract.Three protein fractions were separated from the extract. After coagulation activity test, only the first protein peak with coagulation activity and the activity of the aloe lectin was higher than the crude extract. There were two components in the first protein peak. But it needed further experimental study to identify what was the specific chemical composition of the two components. Conclusion:There were two components found in the lectin of aloe from aloe barbadensis miller.%目的:对库拉索芦荟凝集素进行分离、纯化与初步鉴定.方法:库拉索芦荟经捣碎匀浆、盐析、透析后得到库拉索芦荟凝集素的粗提液;兔血凝集反应测试初步检测粗提液成分;将粗提液进行SDS-聚丙烯酰胺凝胶电泳和葡聚糖G-75凝胶柱层析,对分离组分进行分析;将洗脱液浓缩,再用SDS-聚丙烯酰胺凝胶电泳和葡聚糖G-200凝胶柱层析对组分进行分析.结果:发现库拉索芦荟粗提液可以凝集兔血,证明在粗提液中具有芦荟凝集素成分.对粗提液检测、分离,获得三种蛋白质组分;经凝血活性检测,只有第一个蛋白峰具有凝集活性且凝集效果较粗提液进一步提高.再次分离纯化洗脱液,可知其由两个组分组成.结论:库拉索芦荟凝集素由两种组分组成.