基于SSR标记的黑龙江省绿豆品种遗传多样性分析及指纹图谱构建

摘要

Objective:The project of this study was to analysis the genetic diversity and establish SSR fingerprinting database of mungbean varieties from Heilongjiang province to lay the foundation for breeding and variety identification of mung bean.Methods:The 35 mungbean varieties were used as materials to analyze the genetic diversity and construct DNA fingerprint maps by 14 SSRs.Results:By using 14 pairs of SSR primers,49 alleles were detected out from 35 mungbean varieties,and each pair of primers detected out 2～7 alleles with an average of 3.5.The polymorphic information content values(PIC) ranged from 0.106 to 0.761,with a mean of 0.298.The genetic diversity coefficient among 35 mungbean varieties varied from 0.6966～ 1.0000 with an average of 0.83,and the genetic similarity coefficient of 51.2％ tested materials ranged from 0.82 to 0.86.UPGMA clustering results showed that 35 mungbean varieties were clustered into 3 groups at 0.83 of genetic similarity coefficient.Establishing the SSR fingerprinting database and molecular identification of 35 mungbean cultivars from Heilongjiang province,providing reference for variety identification.Conclusion:The genetic background of mung bean varieties in Heilongjiang was narrow,and the level of genetic diversity was low.SSR fingerprints and molecular identification were constructed using SSR marker technique,which was of great significance for genetic authenticity,identity validation,traceability management and protection of origin of mungbean varieties.%目的:为明确黑龙江地区绿豆品种遗传多样性水平并构建SSR指纹图谱,为绿豆育种研究及品种鉴定奠定基础.方法:基于SSR荧光标记技术,利用筛选得到的14对SSR引物对黑龙江地区35份绿豆品种进行遗传多样性分析及SSR指纹图谱构建.结果:14对SSR引物在35个绿豆品种中共检测到49个等位基因,每对引物检测到2～7个,平均3.5个;多态性信息含量PIC值介于0.106～0.761之间,平均为0.398.参试品种遗传相似系数变幅介于0.6966～1.0000之间,平均为0.894,其中在0.82～0.86之间的遗传相似系数占全部数据的51.2％.在遗传相似系数为0.83的阈值处可将参试材料分为三大类群.构建了35份具有品种特异性的绿豆SSR指纹图谱及分子身份证,具有唯一性,可用于绿豆品种鉴定.结论:黑龙江地区绿豆品种遗传多样性水平不够丰富,SSR指纹图谱具可应用于绿豆品种真伪鉴定.