The commercially available soybean isoflavone powder was used as sample,LJ-Q2 strain withβ-glucosidase was selected and coupled with optimized immobilizedβ-glucosidase.The coupling conditions were optimized by single factor and response surface method.The content of soybean isoflavone glycoside was determined by high performance liquid chromatography(HPLC).The effects of coupled fermentation conditions on the conversion of daidzein were investigated.The results of optimal conditions were as follows:temperature 54 ℃,coupling time 3 h,initial pH7,immobilized enzyme addition amount 7 ％.The absolute quality of soybean isoflavone aglycon was 13.76 mg,the conversion rate of soybean isoflavone aglycon was 76.8％,application of technology development for soybeans glycosides had reference value.%以市售大豆异黄酮粉为样品,选用产β-葡萄糖苷酶的LJ-Q2菌种与优化后的固定化β-葡萄糖苷酶进行耦合发酵,采用单因素、响应曲面法对耦合发酵条件进行优化,高效液相法(HPLC)测定大豆异黄酮苷元,考察耦合发酵条件对大豆苷元转化率的影响.研究结果如下:最优条件为温度54℃,耦合时间3h,初始pH7,固定化酶添加量7％;大豆异黄酮苷元绝对质量13.76 mg,大豆异黄酮苷元转化率为76.8％.对大豆苷制备应用技术开发有参考价值.
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