研究以强酸性阳离子交换树脂为吸附介质,利用色谱技术对低聚木糖液进行分离纯化。通过对不同吸附介质的筛选及不同因素对强酸性阳离子交换树脂对低聚木糖分离效果的影响,确定最适的分离介质及最佳的分离条件。结果表明,强酸性阳离子交换树脂对低聚木糖液具有较好的分离效果,优化后最佳的分离条件为流速1.5 mL/min、进料量为10 mL、进料浓度为30 g/100 mL、操作温度为70℃,低聚木糖液的纯度为95.68%,低聚木糖与单糖实现了较好的分离,为进一步工业化分离低聚木糖奠定了理论基础。%The xylo -oligosaccharide fluid was purified by chromatographic separation technology with strong acid cation exchange resin as adsorption medium.The optimal separation media and the best sepa-ration conditions were determined by comparing different adsorption media and the effects of different fac-tors on the separation of xylo -oligosaccharide.The results showed that xylo -oligosaccharide was well separated by the strong acid cation exchange resin;the optimized separation conditions was as following:flow rate 1.5 mL/min,feed rate 10 mL,feed concentration 30 g/100 mL,operating temperature 70 ℃, the purity was 95.68%,xylo -oligosaccharide was well separated with monosaccharide,which laid a theoretical foundation for the further industrial separation of xylooligosaccharide.
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