肺癌完全性切除术对患者外周血中Th17/Treg细胞平衡的影响

摘要

Objective The aim of this study was to investigate the effect of complete lung cancer resection on the balance of Th17/Treg cells in the peripheral blood of lung cancer patients. Methods Flow cytometry was used to detect the percentage of Th17 and Treg cells in the peripheral blood of 21 patients with lung cancer before and after operation and 21 healthy controls. RT-PCR was used to detect the expression of fork-head/winged helix transcription factor( Foxp3) and retinoic acid-related orphan receptor γt ( RORγt)in PBMC. The plasma levels of interleukin(IL) -17 and transforming growth factor(TGF) -β1 were detected by enzyme-linked immunosorbent assay(ELISA). Results After surgery,the percentage of Th17 cells in the peripheral blood decreased and the percentage of Treg cells increased in patients when compared to the pre -operation ( P <0. 01). After surgery,the expression of RORγt was decreased and the expression of Foxp3 was increased in the CD4 +T cells of patients in comparison with the pre-opera-tion(P<0. 01). After surgery,the expression of IL-17 was decreased and the expression of TGF-β1 was increased in the plasma of patients in comparison with the pre -operation( P <0. 01). In addition,the percentage of Th17 and Treg cells,the expression of RORγt and Foxp3,and the expression of IL-17 and TGF-β in the peripheral blood were increased in preoperative lung cancer pa-tients when compared to healthy controls. Conclusion There is a Th17/Treg imbalance in the peripheral blood of lung cancer pa-tients after complete resection of lung cancer.%目的 探讨肺癌完全性切除术对肺癌患者外周血中Th17/Treg细胞平衡的影响.方法 流式细胞术检测21例肺癌患者术前、术后和21名健康对照者外周血中Th17、Treg细胞的百分比,RT-PCR法检测PBMC中转录因子孤独核受体(Retinoic acid-related orphan receptor γt,RORγt)、叉头翼状旋转转录因子(Fork-head/winged helix transcription,Foxp3)的表达. ELISA法检测血浆中IL-17和TGF-β1 的水平.结果 手术后患者相比术前外周血中Th17 细胞百分比减少,而Treg细胞百分比增加(P<0. 01),手术后患者相比术前CD4+T细胞中RORγt表达量降低,而Foxp3表达量升高(P<0. 01),手术后患者相比于术前血浆中IL-17的表达降低(P<0. 01),而TGF-β1的表达增加( P<0. 01).此外,术前的肺癌患者和健康对照者相比,外周血中Th17和Treg细胞百分比、RORγt和Foxp3表达量以及IL-17和TGF-β表达水平均增加.结论 肺癌完全性切除术后肺癌患者外周血中存在Th17/Treg失衡.