• 主题
高级搜索  >
历史搜索 清空历史
您现在的位置：首页> 中文期刊>肿瘤学>医药卫生>实用肿瘤学杂志

实用肿瘤学杂志

实用肿瘤学杂志

CSTPCD

Practical Oncology Journal
本刊运用现代医学揭示肿瘤发病规律，进行学术交流、沟通信息，促进肿瘤防治工作的发展，鼓励从事肿瘤研究与临床的工作者勇于开拓、创新，为人民健康事业做贡献！
  • 发文量：3762
  • 被引量：590
  • H指数：1
  • 开始收录时间：1989,3(1)
  • CNKI综合因子：0.87
  • 维普期刊影响因子： 1.9
  • 万方期刊影响因子：0.89
  • 创刊时间：1987
  • 国内刊号/CN：23-1212/R
    国际刊号/ISSN：1002-3070
  • 发行周期：双月刊
    邮发代号：14-159
  • 主管单位：黑龙江省卫生厅
    主办单位：黑龙江省、辽宁省、吉林省肿瘤防治办公室
实用肿瘤学杂志-联系信息
  • 主编：曲国蕃
  • 电话：0451-86298293 86623347
  • 邮箱：syzlxzz@126.com
  • 地址：哈尔滨市南岗区保健路6号
  • 邮编：150081
实用肿瘤学杂志-获奖情况
  • • 黑龙江省第七届期刊优秀文章编辑暨第四届期刊装帧设计奖评选活动中荣获一等奖一项、二等奖两项
  • 收录汇总
  • 刊内文献检索
  • 投稿信息

刊期浏览

相关中文期刊

相关中文会议

相关外文期刊

相关外文会议

最新期刊

热门期刊

实用肿瘤学杂志 >2024年第1期
搜索

学科

年度

全选（0）
排序：
共4384条结果
  • 摘要：Objective To detect the level of Twist gene in circulating tumor cells( CTCs )in patients with metastatic breast cancer and evaluate the reaction to treatment of every patient. We tried to discuss the prediction significance of Twist in CTCs to curative effect. Methods We detected the expression of Twist gene in CTCs from 58 metastatic breast cancer( MBC )patients by magnetic activated cell sortingv MACS technology combined with RT - PCR. We evaluated the clinic significance and the curative effect of chemotherapy in MBC patients using Response Evaluation Criteria in Solid Tumors( RECIST ). Results There was no positive expression of Twist mRNA in blood samples from 10 healthy volunteers based on our methods. Among 58 MBC samples, 19/ 58( 32. 8% )were found to express Twist mRNA. In three different breast cancer subgroups,Twist mRNA was expressed more frequently in the triple negative subgroups than in luminal A and Her - 2 positive subgroups( P < 0.001 ). After two cycles of chemotherapy, the overall response rate( ORR )of MBC patients to chemotherapy in Twist mRNA positive group was lower than in Twist mRNA negative group( P <0. 05 ). Conclusion Some CTCs undergo EMT and express epithelial mesenchymal transition gene Twist. CTCs undergoing EMT in breast cancer patients may have a survival advantage because of resistance to chemotherapy. The presence of mesenchymal CTCs predicted resistance to chemotherapy and they maybe one of the reasons of invalid therapy of triple negative breast cancer.%目的 检测转移性乳腺癌患者外周血循环肿瘤细胞上皮间质转化基因Twist的表达水平及化疗疗效,探讨Twist对晚期乳腺癌化疗效果的预测意义.方法 应用免疫磁性分选技术(MACS)联合逆转录-聚合酶链反应(RT-PCR)方法检测实验组(58例转移性乳腺癌患者)和对照组(10例健康志愿者)外周血中循环肿瘤细胞(CTCs)Twist基因的表达.分析其与乳腺癌临床病理学参数的关系,并评估CTCs具有不同Twist基因表达的患者之间治疗效果的差异.结果 在对照组的血液样本中,未检测出Twist mRNA的表达.在实验组的血液样本中,19例(32.8%)Twist mRNA表达阳性,39例(67.2%)Twist mRNA表达阴性.在不同的乳腺癌分子亚型中,三阴性乳腺癌组的Twist mRNA表达明显高于Luminal A组和Her-2阳性组(P＜0.001).根据患者化疗后疗效评估结果分析,Twist mRNA表达阳性组患者对化疗的有效率低于阴性组(P＜0.05).结论 部分循环肿瘤细胞表达Twist基因.发生上皮间质转化的循环肿瘤细胞有更强的抵抗化疗药物的能力,是三阴性乳腺癌疗效不佳的原因之一.
  • 摘要：GRP78 as an endoplasmic reticulum molecular chaperones has been found to be assoiated with tumorigenesis. GRP78 has high expression in tumortissues, which promoting the growth and metastasis of tumor. Expression of GRP78 may become an index to predict tumor prognosis. It has significance in the treatment of tumor.%葡萄糖调节蛋白78(GRP78)作为一种内质网分子伴侣,与肿瘤的发生、发展十分密切,GRP78在肿瘤组织中有较高的表达,促进肿瘤细胞的增长与转移.有实验证实,GRP78的表达水平可以作为预测肿瘤预后的指标,并在探索肿瘤的治疗中有一定的参考意义.
  • 摘要：Objective The study was to construct and identify the eukaryotic expression plasmid pEGFP - C3/Smad3, and then transfect it into MCF - 7 breast cancer cells. To lay a foundation for further research on the biological function of Smad3 in the inhibition of breast cancer growth mechanism. Methods The cDNA was extracted from MCF - 7 breast cancer cells by RT - PCR, the Smad3 gene fragment was amplified and extracted by PCR,and the amplified products were inserted into pEGFP - C3 eukaryotic expression vector then sequenced and identified by restrictive endonuclease digestion. The constructed recombinant plasmid was transitorily transfected into the MCF - 7 breast cancer cells and the expression of Smad3 was observed by inverted fluorescence microscope,RT -PCR technology and westen blot. Results The experiment was successfully constructed the eukaryotic expression plasmid pEGFP - C3/Smad3 and transiently transfected into MCF - 7 cells. Conclusion The eukaryotic expression plasmid pEGFP - C3/Smad3 was transiently transfected into MCF - 7 cells. Smad3 gene and protein levels can be significantly raised.%目的 构建pEGFP-C3/Smad3真核表达质粒,并进行鉴定.为进一步研究Smad3在抑制乳腺癌生长机制中起到的作用提供实验基础.方法 利用RT-PCR方法从MCF-7乳腺癌细胞中获得cDNA,应用PCR方法扩增、提取 Smad3的基因片段,酶切后与pEGFP-C3真核表达载体连接,构建pEGFP-C3/Smad3真核表达质粒,进行测序、酶切鉴定,表明质粒构建成功.将构建的质粒瞬时转染至MCF-7乳腺癌细胞中,通过荧光倒置显微镜观察、RT-PCR技术及Westen blot技术鉴定转染是否成功.结果本实验成功构建了pEGFP-C3/Smad3真核表达质粒.结论 pEGFP-C3/Smad3真核表达质粒瞬时转染到MCF-7细胞中,可使Smad3在基因与蛋白水平的表达显著上调.
  • 摘要：Objective To evaluate inhibition effecton proliferation of SCG - 7901 cell in different sequences combination of Cetuximab with either oxaliplatin, funaioniding or Capecitabine. To explore the optimal treatment schedule of combination of Cetuximab and cytotoxic drugs. Methods Increasing concentration of Cetuximab( 5 ~ 500 mg/L ) combined with increasing concentration cytotoxic drugs was administrated to human gastric cancer cell lines SCG - 7901 with different sequences. The proliferation inhibition effect on SCG - 7901 cell was detected with CCK - 8 assay. The IC50 and CI of Cetuximab and either cytotoxic drugs were calculated. Results The combination of a cytotoxic drug with Cetuximab caused different antiproliferative effecs on SCG -7901 cells depending on different treatment sequences. The respective IC50 of Cetuximab and cytotoxic drugs decreased when Cetuximab followed by chemotherapy, while the CI values were all >1 , which indicated an antagonistic interaction. "In contrast, when chemotherapy was followed by Cetuximab, the respective IC50 of drugs decreased more remarkably and the CI values were all < 1, which indicated a significant synergistic antiproliferative activity. Conclusion Monoclonal antibody and cytotoxic drugs to SGC -7901 cell lines is antiproliferative activity. The optimal treatment sequence is chemotherapy followed by Cetuximab. It can obtain obviously synergistic activity and conduce to less doses and milder side effects in clinical application.%目的 探讨不同序贯的西妥昔单抗(Cetuximab,C225)与化疗药物联合对人胃腺癌细胞株SGC-7901的增殖抑制作用,寻找治疗胃癌的新方法、新配方.方法 选用浓度递增的Cetuximab和化疗药物奥沙利铂、氟尿嘧啶、卡培他滨,分别按照不同给药方式单药、序贯作用于SGC-7901细胞,采用细胞计数试剂盒检测不同给药方式对SGC-7901细胞的增殖抑制作用,计算不同序贯给药时Cetuximab和化疗药物的半数抑制浓度(IC50)的变化,及其相互作用的协同系数(CI).结果 Cetuximab和化疗药物联用对SGC-7901细胞的增殖抑制作用受给药序贯影响:Cetuximab先于化疗药物给药,两者的IC50较单用时降低,但CI值均大于1,两者之间为拮抗效应;Cetuximab后于化疗药物给药,两者的IC50较单用时降低更为明显,且CI值均小于1,两者之间具有较明显的协同效应.结论 Cetuximab联合化疗药物对胃癌细胞SGC-7901的增殖具有协同抑制作用,化疗药物先于Cetuximab的序贯给药方式能获得明显的协同效应.
  • 摘要：As the evolutions of pathogenesis and oncotherapy on oncology, great progression has been made in treating with primary hepatocellular carcinoma( HCC ). Recent years, argon - helium cryoablation, which is a new therapeutic method,has been extensivly used in clinical. However,after treating primary hepatocellular carcinoma by argon - helium cryoablation, how to evaluate effectiveness economically and practically is still a puzzle in clinical application. In this review,we will approach the related research from home and aboard.%随着肝癌发生机制研究的深入及肿瘤治疗技术的进步,对肝癌的治疗也有了一定的进展.近年来,氩氦刀治疗肝癌作为一种新的治疗方法,在临床上逐渐得到推广应用.然而肝癌氩氦刀治疗后,如何经济又实用的判断其治疗疗效,一直是困扰临床应用的实际问题.本文拟就国内外相关研究进展进行探讨.
  • 摘要：Early screening and monitoring recurrence of hepatocarcinoma postoperative were still the most challenging works in clinical work, although there were AFP, CA199 , AFU, GGT, Human telomerase reverse transcriptase mRNA and heat shock protein as tumor markers in clinical application. But,each tumor marker has some disadvantage. Specificity is not strong,which often needs to unite detection for improving the accuracy of the diagnosis. Abnormal glycosylation of glycobiology has a close relationship with tumor. So, the study of abnormal glycosyl has become hot issue in recently years. N - glycan is a kind of abnormal glycosylation. Thus, the detection of specific N - glycan may become dynamic changing index in screening and monitoring hepatocarcinoma.%肝癌的早期筛查和术后复发的监测仍是临床工作中的难点,虽然已经有甲胎蛋白、CA19-9、AFU、GGT、人端粒酶反转录酶mRNA、热休克蛋白等肿瘤标记物在临床上广泛应用,但是每种肿瘤标记物都存在一定弊端,特异性不强,往往需要联合检测才能提高诊断的准确性.糖生物学中的异常糖基化被认为与肿瘤的发生发展有密切关系,因此研究异常糖基化成为近年来的研究热点.N-糖链是异常糖基化的一种,检测特异性的N-糖链有望成为筛查及监测肝癌患者变化的动态指标.
  • 摘要：目的 转基因小鼠是进行肿瘤研究的良好材料,采用化学诱导剂在绿色荧光蛋白转基因C57BL/6小鼠(GFP-C57BL/6)上建立肿瘤模型.方法 本研究采用苯并(α)芘对GFP-C57BL/6进行前胃癌的诱导实验.结果 苯并(α)芘处理后30周,小鼠前胃肿瘤发生率达到100%,且不会照成对照小鼠胃部损伤.结论 采用苯并(α)芘可以在GFP-C57BL/6小鼠成功建立肿瘤动物模型.%Chemically induced tumor model is one of common techniques for cancer research. The mouse of transgenic green fluorescent protein gene is also a good material. In the research, B( a )P was used to induce forestomach neoplasia in green fluorescent protein transfected C57BL/6( GFP - C57BL/6 )mouse. The results show that the B( a )P can be induce forestomach neoplasia successfully. The tumor incidence reached 100% when the GFP - C57BIV6 was treated with B( a )P. It could be used for a inducer in GFP - C57BL/6 forestomach neoplasia model.
  • 摘要：Objective To study the expression of breast cancer stem cell - like markers CD44+ CD24 -/low in basal - like breast carcinoma( BLBC )and their relation with the prognosis of BLBC. Methods We classified breast carcinomas into 4 groups: luminal A, luminal B/C , Her - 2 over - expressing and triple - negative subtype with the expressions of ER,PR and Her - 2, respectively. We classified triple - negative subtype into 2 groups:BLBC and normal breast - like group with the expression of CK5/6 and EGFR, respectively. Immunohisto-chemical( Envision method )was performed to all of the 5 groups, with the selection of antibodies for CD44 and CD24. Results ( 1 ) triple - negative group were 60 cases in all, expressed CK5/6 and EGFR in 41cases ( 68. 3% ),which was identified as BLBC;low expressed CK5/6 and EGFR in 19 cases( 31.7% ),which identified as the normal breast - like group. ( 2 )32 cases expressed CD44+ CD24-/low phenotype in 41 cases of BLBC78.0%( 32/41 ),compared with luminal A group of 37.9%( 11/29 ),luminal B of25.9%( 7/27 ),Her-2 over - expression group of 17. 2%( 5/29 )and normal breast - like group of 26. 3%( 5/21 )in BLBC( P < 0. 05 ). ( 3 )ln all cases of breast cancer which can be evaluated in 145 cases with CD44 + CD24 -/low immun - phenotype, Ki - 67 index was higher and statistically significant! P < 0. 05 ). Conclusion The expression of human breast cancer stem cell markers CD44 + CD24-/low in BLBC was significantly higher than other types of breast cancer. Then, we can suppose an association between the prognosis of BLBC and the presence of CD44+ CD24 -/low cell.%目的 探讨乳腺癌干细胞样标志物CD44+CD24-/low在基底样乳腺癌(basal-like breast carcinoma,BLBC)中过表达与BLBC恶性预后的相关性.方法 在乳腺癌基因表达分型的基础上,根据雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(Her-2)免疫表型的表达选取乳腺癌组织四组:管腔A组、管腔B/C组、Her-2高表达组及三阴性组;对三阴性组检测CK5/6、EGFR,分为正常乳腺样型和BLBC型两组;对上述5组进行免疫组化Envision法染色,选用抗体为CD44、CD24,观察CD44+CD24-/low表型表达并比较BLBC组与其它各组的差异.结果 (1)三阴性组共60例,CK5/6和或EGFR阳性者41例(68.3%),确定为BLBC;CK5/6、EGFR阴性者19例(31.7%),确定为正常乳腺样组;(2)CD44+CD24-/low表型在BLBC组中占78.0%(32/41),相对于管腔A组37.9%(11/29)、管腔B组25.9%(7/27)、Her-2高表达组17.2%(5/29)、正常乳腺样组26.3%(5/21),表达增高并具有显著性(P＜0.05);(3)所有150例乳腺癌中(可评价145例)具有CD44+CD24-/low免疫表型者其Ki-67指数增高相对于非CD44+CD24-/low表型具有统计学差异(P＜0.001).结论 BLBC型乳腺癌表达乳腺癌干细胞样标志物CD44+CD24-/low显著高于其它各型乳腺癌,CD44+CD24-/low与BLBC独特的恶性生物学行为相关.
  • 摘要：Objective To investigate the correlation between ultrasonic features and Ki - 67 of breast cancers. Methods The expression of Ki -67 in tumor tissue in 80 cases with breast cancers who were examined by ultrasonography in preoperation were detected by immunohistochemical technique. Results In 80 cases of breast cancers,the expression of Ki -67 were positively correlated to tumor size,with tiny calcifications,with rich bloodstream and lymphatic metastasis! P < 0. 05 ); the pression of Ki - 67 was not related to mass edge and posterior echo attenuation( P > 0. 05 ). Conclusion The ultrasonographic characteristics of breast cancer show correlation with the expression of Ki -67. Both combination of diagnosis and treating early breast cancer have some clinical significance.%目的 探讨乳腺癌超声声像图特征与Ki-67表达的相关性及临床意义.方法 对术前应用超声检查的80例乳腺癌患者术后的肿瘤标本应用免疫组化法检测Ki-67表达情况,并分析与超声声像图之间的相关性.结果 在80例乳腺癌患者中,Ki-67表达与肿块最大直径、有无微小钙化、血流丰富程度及淋巴结转移呈正相关(P＜0.05);与肿块边界、后方回声无明显相关性(P＞0.05).结论 乳腺癌超声声像图特征与Ki-67表达具有一定的相关性,两者联合检测对于早期乳腺癌的诊断及治疗有一定的临床意义.
  • 摘要：Primary hepatocellular carcinoma( PHC )is one of the most common malignancies worldwide. Because PHC usually presents as an intermediate or advanced disease and occurs in the background of liver cirrhosis , most patients are not suitable for treatment with curative intent. Thus, effective systemic chemotherapy or molecular targeted therapy is required. The purpose of this article is to review the current status, progress of systemic chemotherapy and molecular targeted therapy of PHC.%原发性肝癌(primary hepatocellular carcinoma,PHC)是世界最常见的恶性肿瘤之一.因其发现时往往已是中晚期,并伴随有肝硬化改变,大部分病人难以进行根治性治疗,因此有效的全身化疗或分子靶向治疗成为必要的治疗手段.本文就晚期PHC的全身化疗及分子靶向治疗的现状及进展做一综述.
  • 摘要：Objective To investigate the correlation of the telomerase activity and radiosensitivity of surviving progeny from different doses irradiated cervical carcinoma cell. Methods The HeLa cells were irradited with 0,2,6,10 Gy of X -ray for three times and the surviving progeny were subculture. There were four groups: HeLa, HeLa - 2 , HeLa - 6 , HeLa - 10. The telomerase activity was measured by the polymerase chain reaction -based telomeic repeat amplification protocol coupled with ELISA. And the expression of the hTERT mRNA were detected by the RT - PCR assay. The radiosesitivity index was examined by clone formation assay. Results The HeLa cells were treated by different doses of irradiation. The telomerase activity of HeLa group HeLa - 2 group HeLa -6 group was( 1. 54 ±0. 01 ),( 1. 87 ±0. 01 ),( 2. 03 ±0. 02 )and the expression of hTERT mRNA was increased with the irradiation dose and was( 0. 44 ± 0. 03 ) ,( 0. 53 ± 0. 04 ),( 0. 74± 0. 07 ). Each parameter had significant differences among the survival progenies! P < 0. 05 ). But the HeLa - 10 group was( 1. 02 ±0. 024 )and (0.21 ±0. 02 )significantly reduced. Each group's radiosensitivity was declined with the different irradiation dose. The telomerase activity and the expression of the hTERT mRNA was negatively correlated with radiosensitivity of each groups( r = - 0. 927 , P < 0. 01: r = -0. 904 , P < 0. 01 ). Conclusion Radiosensitivity of the surviving progeny correlates with the telomerase activity and the expression of hTERT.%目的 通过检测不同剂量照射宫颈癌细胞后生长特性及端粒酶逆转录酶的表达,探讨其与放射敏感性之间的关系.方法 将同源的HeLa细胞分为四组,分别给予多次0Gy、2Gy、6Gy和10Gy的X射线照射,建立不同剂量的放射耐受模型,用TRAP-ELISA分别检测HeLa组、HeLa-2组、HeLa-6组和HeLa-10组端粒酶的活性,用RT-PCR检测其端粒酶hTERT的相对表达量,克隆形成实验检测其放射敏感参数α、β及SF2.结果 HeLa组、HeLa-2组、HeLa-6组端粒酶活性分别是1.54±0.01、1.87±0.01、2.03±0.02,各组间的差异具有统计学意义(P＜0.05);hTERT mRNA的相对表达量分别为0.44±0.03、0.53±0.04、0.74±0.07,表达量明显升高且各组间的差异具有统计学意义(P＜0.05);但HeLa-10组端粒酶的活性(1.02±0.02)及hTERT mRNA的相对表达量(0.21±0.02)都明显降低,与以上三组的端粒酶活性及hTERT mRNA的相对表达量各组的差异均具有统计学意义(P＜0.01).四组细胞克隆形成率降低,放射敏感性依次降低.并且HeLa组、HeLa-2组、HeLa-6组各组端粒酶活性与各组细胞的放射敏感性呈负相关(r=-0.927,P＜0.01),以上三组的hTERT mRNA的相对表达量与各组细胞的放射敏感性亦呈负相关(r=-0.904,P＜0.01).结论 在一定剂量限度内,经过不同剂量的射线照射的宫颈癌HeLa细胞系,其端粒酶活性、hTERT mRNA的相对表达与其放射敏感性存在一定相关性.
  • 摘要：Objective To investigate the difference of expressions of ER, PR and Her - 2 status between primary lesion and metastatic axillary lymph nodes of TNBC, and the relationship between the variability and prognosis of patients. Methods The expressions of ER, PR and Her - 2 were detected in primary lesion and metastatic axillary lymph nodes in 45 cases of TNBC by immunohistochemistry, and then analyzed the relationship between modifications of these markers and live time by Kaplan - Meier survival curves. Results Compared with the primary lesion of TNBC ,48. 9% patients'axillary lymph node metastases of ER,PR and Her -2 still expressed negative , but 51.1% patients had at least one marker positive expression, means immunohistochemical staining of axillary lymph nodes were different to primary lesion, with a significant difference between them. The expression variability was significant correlated with live time( P = 0. 013 ). The live time of the group which immunohistochemical staining of axillary lymph nodes similar to the primary lesion of TNBC was shorter than the group that was different to primary lesion( P = 0. 010 ), and there had statistical significance by Log Rank method( P = 0. 048 ). Conclusion In primary lesion and metastatic axillary lymph nodes of TNBC ,the expressions of ER,PR and Her -2 were difference. And the variability was correlated with life time of patients.%目的 探讨雌激素受体、孕激素受体、人表皮生长因子受体-2在三阴性乳腺癌原发灶和腋淋巴结转移灶中表达有无差异及其与患者预后的相关性.方法 运用免疫组化法分别检测45例TNBC原发灶及腋淋巴结转移灶中ER、PR、Her-2的表达,用Kaplan-Meier生存曲线分析TNBC腋淋巴结转移灶中这些指标的变化与生存时间的关系.结果 与TNBC原发灶相比,腋淋巴结转移灶中ER、PR、Her-2仍表达阴性的患者占48.9%,而至少有一项指标表达阳性的患者占51.1%,即与原发灶表达不一致,差异具有统计学意义,且这种表达差异与生存时间存在相关性(P=0.013).腋淋巴结转移灶免疫组化表达与原发灶一致组的生存时间要短于不一致组(P=0.010),用Log Rank法检验具有统计学意义(P=0.048).结论 ER、PR、Her-2在TNBC原发灶和腋淋巴结转移灶中的表达存在不一致现象,且这种不一致现象与生存时间具有相关性.
  • 摘要：目的 探讨BI-RADS 3-4级乳腺X线表现与病理诊断的相关性.方法 回顾分析75例患者共88个病灶的X线钼靶评估为BI-RADS3-4级病灶的影像征象,分别统计各影像征象与良恶性之间的关系,运用Logistic回归进一步分析.结果 病变类型、边缘、形态、钙化形态及淋巴结情况与病变良恶性之间均有统计学差异(P＜0.05).腺体型、密度、毛刺、病灶最大径、钙化有无和钙化分布与病变良恶性之间均无统计学意义(P＞0.05).Logistic回归分析进一步得出边缘的OR(1.859)取值最大,95%可信区间(95%CI)1.032~3.347,P=0.039,差异有统计学意义.结论 BI-RADS 3-4级X线表现中病灶的类型、边缘、形态、钙化形态及淋巴结情况对于良恶性的鉴别有意义,其中病灶边缘与良恶性分组显著相关.%Objective To investigate the relationship of mammographic features and histological findings in BI - RADS 3 and 4. Methods 75 patients with 88 cases were studied, which were categorized as BI - RADS 3 and 4 ,retrospectivly. The correlation between mammographic features and pathological findings was analyzed statistically. Logistic regression was used to improve the analytical results. Results There was difference between benign and malignant tumors:lesion type,margin,form,calcification morphologic and lymph node status. Logistic regression further showed that there was significant difference of margin with the odds ratio( OR )l. 859,95% confidence interval( 95% CI )l. 032 ~ 3. 347 ,and confidence coefficient P =0. 039 ). But,no difference was found in different mammary gland type, density, spiculation, different diameter groups, calcification and its distribution ( all with P >0. 05 ). Conclusion BI - RADS category 3 and 4 : differences exist in category, margin, form, calcification morphologic and lymph node status between benign and malignant tumors,especially in the margin of a lesion.
  • 摘要：Nasopharyngeal Carcinoma( NPC )is one of the most common malignant tumors in our country. The number of patients accounts for 80% in the world. Radiation therapy is the first choice for NPC patients. The technique of radiation therapy develops from conventional radiation therapy to precision radiation therapy. It develops from three - dimensional conforms! radiotherapy( 3DCRT )to intensity, which modulates radiotherapy ( IMRT )and the emergence of the image guided radiotherapy( IGRT ). These techniques not only improve the ac-curacy of radiation therapy ,but also reduce the damage of radiation therapy. They gradually become the main techniques of modern radiation therapy. This parcel mainly introduces the application, the curative effect and the research status of the above techniques in NPC treatment.%鼻咽癌是我国最常见的恶性肿瘤之一,全世界80%的鼻咽癌发生在我国.放射治疗是鼻咽癌首选治疗手段,现代的放射治疗技术已从常规放疗发展至精确放疗,从三维适形放射治疗至适形调强放射治疗再到图像引导放射治疗的出现,不但提高了放疗的准确性,而且大大减轻了放疗的副反应,逐渐成为现代放疗的主流技术.本文主要介绍了以上技术在鼻咽癌治疗中的应用、疗效及国内外的研究现状.
  • 摘要：目的 研究右旋柠烯(D-limonene)对人结肠癌细胞(LS174T)中信号传导分子ERK1/2、Akt及其磷酸化蛋白(p-ERK和p-Akts蛋白)表达的影响.方法 采用四甲基偶氮唑盐(MTT)方法检测右旋柠烯对LS174T细胞增殖的抑制作用;采用Western blot方法检测不同浓度右旋柠烯对LS174T细胞中ERK1/2、Akt及其磷酸化蛋白表达情况的影响.结果 右旋柠烯作用LS174T细胞48h后,呈剂量依赖性抑制细胞增殖;Western blot结果显示右旋柠烯对细胞中ERK1/2、Akt蛋白的表达没有明显影响,但显著下调p-ERK1/2蛋白和p-Akts的表达.结论 右旋柠烯抑制LS174T细胞增殖可能与其抑制p-ERK1/2和p-Akts表达有关.%Objective To investigate the effect of D - limonene on the expressions of p - ERK and p -AKTs in human colon cancer cell line LS174T. Methods The effect of D - limonene on the cell proliferation was measured by methyl thiazolyl tetrazolium( MTT ), and the expression of ERK1/2, Akt and their phosphorylated proteins( p - ERK and p - AKTs )were detected by Western blot. Results D - limonene inhibited the proliferation of LS174T cell in a dose dependent manner, because the LS174T cell were exposed to D - limonene for 48 hours. D - limonene did not influence the expressions of ERK1/2 and Akt, but it significantly decreased p -ERK1/2 and p - Akts expressions. Conclution It is suggested that D - limonene could inhibit proliferation of LS174T cell via inhibiting the p - ERK1/2 and p - Akts pathway.
  • 摘要：Objective To study the effect of Angiotensin II on cell proliferation and the activity of matrix metalloproteinase in breast cancer cells. Methods MCF - 7 cells were cultured in vitro and stimulated with Angiotensin Ⅱ and/or Losartan. The viability of cells was tested by MTT assay. We used RT - PCR to detect the mRNA level of MMP - 2 , MMP - 9 ,TIMP - 1 and TIMP - 2. The activity of MMP - 2 and MMP - 9 were measured by gelatin zymography. Results Angiotensin II stimulated the growth of breast cancer cells in a dose - and time -dependent manner. Moreover,the expressions of MMP -2 and MMP -9 mRNA were significantly up -regulated by Angiotensin Ⅱ. The expressions of TIMP - 1 and TIMP - 2 mRNA were significantly down - regulated by Angiotensin Ⅱ. In addition, Angiotensin II significantly enhanced the activity of MMP -2 and MMP -9. Angiotensin Ⅱ had a maximal response with 100 nM( P < 0. 05 ). Losartan significantly decreased the effect of Angiotensin Ⅱ on matrix metalloproteinase. Conclusion In summary,our study can support the concept that Angiotensin Ⅱ/ Angiotensin Ⅱ type 1 receptor( AT1R )signaling can regulate the expression of matrix metalloproteinase. Losartan can significantly decrease the effect of Angiotensin Ⅱ on matrix metalloproteinase. Therefore, targeting Angiotensin Ⅱ / Angiotensin Ⅱ type 1 receptor( AT1R ) signaling could be a novel therapeutic for breast cancer.%目的探讨血管紧张素Ⅱ(Angiotensin Ⅱ)对乳腺癌MCF-7细胞基质金属蛋白酶表达和活性的影响.方法 体外培养乳腺癌MCF-7细胞,经血管紧张素II及血管紧张素Ⅱ 1型受体阻断剂(氯沙坦)处理后,用MTT法检测细胞增殖能力的变化,RT-PCR法检测MMP-2、MMP-9、TIMP-1和TIMP-2 mRNA的表达情况,明胶酶谱法检测MMP-2和MMP-9酶活性的变化.结果 血管紧张素Ⅱ可以促进乳腺癌细胞的生长,并且呈现剂量和时间依赖性;血管紧张素Ⅱ可上调MMP-2和MMP-9 mRNA的表达,抑制TIMP-1和TIMP-2 mRNA的表达,同时增强MMP-2和MMP-9酶的活性,其中100nM的血管紧张素Ⅱ作用最显著(P＜0.05).用氯沙坦预先处理细胞可以显著抑制血管紧张素Ⅱ对基质金属蛋白酶表达和活性的调节作用.结论 血管紧张素Ⅱ通过与血管紧张素Ⅱ 1型受体结合来调节乳腺癌细胞基质金属蛋白酶的表达,其受体阻断剂(氯沙坦)可以显著抑制血管紧张素Ⅱ的作用.因此血管紧张素Ⅱ/血管紧张素Ⅱ1型受体有可能成为乳腺癌治疗的靶点.
  • 摘要：Objective To discuss on the BALB/C of lung tissue in mice after irradiation, TNF - alpha and IL - 1 alpha release characteristics of cytokines in the lung and liver. Methods Irradiation of male BALB/C mouse 30 was randomly assigned to a group( R )and the control group( C ). R group:24 / group, C group:6 / group ,6MV - X - Ray 25 Gy chest irradiation; two groups of mice after exposure to 1, and 2 ,and 3 months were executed using immunohistochemical methods for detection of irradiation in mouse lung and liver tissue of TNF -α and IL - 1α expression levels. Results R group of TNF - a and IL - la expressions in lung and liver levels of exposure to 1, and 2, and 3 months of significantly higher C( P < 0. 01 )and 2 months after the expression of the highest. Conclusion Lung tissues after exposure TNF - α and IL - lα expressions in lung and liver levels are significantly increased. Exposure 2 months in mice TNF-α and IL -1α expressions are particularly significant.%目的 探讨BALB/C小鼠肺组织照射后,TNF-α和IL-1α细胞因子在肺和肝脏的释放特点.方法 雄性BALB/C小鼠30只随机分配到照射组(R)和对照组(C).R组:24只/组,C组:6只/组,照射组用6MV-X射线单次25Gy胸部照射;两组小鼠在照射后1个月、2个月、3个月被处死,采用免疫组化方法检测照射小鼠肺和肝脏组织中TNF-α和IL-1α表达水平.结果 R组的TNF-α和IL-1α在肺和肝脏的表达水平在照射后1个月、2个月、3个月明显高于C组(P＜0.01),在2个月后表达最高.结论 肺组织照射后TNF-α和IL-1α在肺和肝脏的表达水平明显增高,在照射后2个月的小鼠中TNF-α和IL-1α表达尤为显著.
  • 摘要：LAPTM4B基因是新近发现的癌症相关基因,具有癌基因的特性.其mRNA和编码的LAPTM4B-35蛋白在许多肿瘤中显著上调,并与肿瘤细胞的分化、增殖、侵袭、多药耐药等密切相关.近年来研究较多的是LAPTM4B基因多态性与肿瘤患者预后的关系.现就其结构、生物学功能、基因多态性与肿瘤的易感性以及对肿瘤患者预后影响等方面作一综述.%LAPTM4B gene is a newly discovered cancer - related gene, which has the characteristics of the oncogene. Its mRNA and encoded LAPTM4B - 35 protein were significantly upregulated in many tumors. LAPTM4B gene is closely related to tumor cell differentiation, proliferation, invasion and multidmg resistance. In recent years, the research focused on its relationship with the prognosis of cancer patients and genetic polymorphisms and cancer susceptibility. Now, we are going to cany on the preliminary discussion with respect to its structure, biological function, genetic polymorphism and tumor susceptibility, prognosis and other aspects.
  • 摘要：目的观察常规剂量分割照射模式下单次照射时间延长对肺癌细胞系H520放射生物效应的影响.方法 (1)肺鳞癌细胞株H520离体培养,分组进行照射,利用克隆形成实验计算细胞存活比率(SF);(2)细胞给予总剂量为2Gy、4Gy、6Gy、8Gy的照射,并按照每日单次照射时间的不同分为A组(照射2分钟组)、B1组(照射10分钟组)、B2组(照射30分钟组).观察延长照射时间对肺鳞癌细胞株H520存活比率的影响.结果 伴随单次照射总时间的延长,实验细胞的存活比率逐渐提高,接受照射总剂量为8Gy的H520细胞株,A组细胞存活比率为1.9%;B1组和B2组存活比率分别为2.35%和3.42%.A组与B2组之间的差异具有统计学意义(P＜0.05).结论 常规剂量分割模式下延长单次照射时间,显著降低了放射治疗对H520细胞的生物效应.%Objective The purpose of this study was to observe the radiobiological effects of lung squamous cancer cell line H520 irradiated over prolonged single fraction delivery time in the conventional dose fractionated mode. Methods (1)The H520 cell lines were cultured in vitro, and then irradiated by groups. Its survival fractions(SF)were calculated with standard clonogenic assays.( 2 )The total doses of 2 Gy,4 Gy,6 Gy,8 Gy were given to the cells,which were divided into groups by different single fraction delivery time:group A( group irradiated for 2 mins ),group Bl( group irradiated for 10 mins ),and group B2( group irradiated for 30 mins ),and observed the impact on the survival fractions of H520 cell line with prolonged fraction delivery time. Results The cell surviving fractions increased when the interfraction interval was longer. The values of SF 8 Gy were 1. 9% in group A,2.35% in group B1,and increased to 3. 42% in group B2 ,respectively. The difference between group A and group B2 was statistically significant(P<0.05 ). Conclusion The prolonged fraction delivery time would significantly decrease the radiobiological effects of lung squamous cancer cell line H520 in the conventional dose fractionated mode.
  • 摘要：Objective To compare PMBC and invasive ductal carcinoma( IDC ) and to reliably assess the biologic behavior of PMBC. Methods A total of 610 patients who met the inclusion criteria were identified from 1993 to 2011 ;494 patients had pure IDC and 116 had PMBC. Clinical and biologic features, overall survival and disease - free survival( DFS )were compared for both groups. Results PMBC had favorable characteristics including lower rate of lymph node positivity, lower stage, higher expression of ER and PR, less Her - 2 overex-pression and less chemotherapy after surgery. Patients with PMBC had better 5 - year OS and DFS than patients with IDC( P =0. 036 and 0. 023 , respectively ). Multivariate analysis revealed that ER status was statistically significant prognostic factor for survival. Conclusion PMBC has a favourable histological characteristics and prognostic result. The ER status is the most important single prognostic factor.%目的评估单纯性乳腺粘液癌(Pure mucinous breast carcinoma,PMBC)的生物学行为.方法 对1993-2011年间的610名满足入组条件的患者进行研究,其中494名为浸润性导管癌(Invasive ductal carcinoma,IDC),116名为PMBC.对临床生物学特点、总生存(Overall survival,OS)和无病生存率(Disease-free survival,DFS)进行比较.结果 PMBC患者淋巴结阳性率低,TNM分期低,ER,PR表达水平高,Her-2表达水平低,进行术后化疗患者的比例较少.PMBC患者5年总生存率和无病生存率均高于IDC患者(P值为0.036和0.023).多因素分析结果表明ER状态是影响的预后性因素.结论 PMBC具有良好的组织学特征和预后,其中ER表达情况是影响预后的最重要因素.
  • 摘要：Arsenic trioxide( ATO )is presently one of the most effective single agent in the treatment of a-cute promyelocytic leukemia( APL ). ATO impressivly increases the complete remission rate and prolongs survival of patients with APL with only mild and transient adverse effects. The mechanisms of ATO mainly include promoting differentiation,inducing apoptosis,accumulating reactive oxygen species,and eliminating leukemic - initiating cells. And synergistic actions in introduction of apoptosis and differentiation between ATO and all - trans - retino-ic acid have been shown. In relapsed APL, ATO - based salvage therapy has been able to induce long - lasting remissions and possible cure in 50% -81% of patients. The high anti - leukemic efficacy and the favorable toxicity profile of ATO were also shown in newly diagnosed APL,because high remission and cure rates could even be induced by ATO alone. Experimental research and clinical studies have established ATO as an important candidate for the further improvement of APL therapy.%三氧化二砷(Arsenic trioxide,ATO)是目前单药治疗急性早幼粒细胞白血病(Acute promyelocytic leukemia,APL)最有效的药物之一,可明显提高APL的完全缓解率,延长患者的生存期,且不良反应较轻.ATO的作用机制主要为诱导APL细胞分化与凋亡、促使细胞内活性氧水平升高和去除白血病启动细胞等,并且在诱导分化和凋亡方面与全反式维甲酸起协同作用.对于复发性APL,以ATO为基础的挽救疗法能够诱导APL长期缓解并且50%~81%的患者可能治愈.在初发APL中,ATO表现出抗白血病的高效性和低毒性,使APL获得较高的缓解率和治愈率.因此,对ATO进行深入的基础及临床研究将为进一步优化ATO对APL的治疗提供重要的参考.
  • 摘要：Cellular senescence is broadly defined as the physiological program of terminal growth arrest. Cellular senescence could be triggered by short chromosome telomeres, activated oncogene, as well as cell stress, and mediated by p53 and pl6/pRB tumor suppressor pathways. Treatment - induced senescence, which has both similarit with or difference from replicative senescence of normal cells, was shown to be one of the key determinants of tumor response to therapy in vitro and in vivo. Elucidation of the genes and regulatory mechanism which determines different aspects of tumor senescence makes it possible to design new therapeutic approaches for improving the efficacy and decreasing the side effect of cancer therapy. The elucidation of the biological aspects of tumor cell senescence offers the plausible approach for the development of novel therapeutic strategies, which may stop the growth of tumor cells.%细胞衰老是指细胞在外在微环境变化或内在特定基因表达与失活等因素作用下脱离细胞周期并不可逆地丧失增殖能力后进入的一种相对稳定的状态,能够被端粒的变化或不同形式的胁迫所触发.类似于癌基因诱导凋亡,多种不同癌基因也可以引起细胞衰老.细胞衰老依赖的生长停滞主要是通过p53和p16/pRB肿瘤抑制信号通路建立和维持的.从生物学功能方面看,细胞衰老与肿瘤发生及治疗密切相关.基因操作或用化疗药物等处理可以诱导某些肿瘤细胞衰老,阐明这一过程的发生机制有助于癌症治疗策略的发展.对于肿瘤发生过程中的细胞衰老仍为生物学研究领域的热点.
  • 摘要：Objective To determine the effect of phenolic alkaloids of menispermum dauricum on the gastric cancer cell SGC -7901 proliferation. Methods We studied the effect of phenolic alkaloids of menispermum at different concentrations and different times on the gastric cancer cell SGC - 7901 proliferation by MTT and trypan blue methods. Results PAMD can obviously inhibit SGC - 7901 cell proliferation, and is dose - and time - dependent relationship with extended periods of concentration increase. Its role and inhibition rate was on the rise. Conclusion PAMD may have some therapeutic effect on gastric cancer.%目的测定蝙蝠葛酚性碱对胃癌细胞SGC-7901增殖的影响.方法通过MTT法和台盼兰法,观察蝙蝠葛酚性碱在不同浓度,不同时间条件下,对胃癌细胞SGC-7901增殖作用的影响.结果 蝙蝠葛酚性碱能明显抑制SGC-7901细胞的增殖,并呈剂量和时间依赖关系,随浓度增大和作用时间延长其抑制率呈上升趋势.结论 蝙蝠葛酚性碱对胃癌可能具有一定治疗作用.
  • 摘要：Objective To summarize the etiology, treatment and preventive experience of the chylous fistula after radical resection of colon carcinoma plus D3 lymph node dissection. Methods 628 cases of the chylous fistula after radical resection of colon carcinoma plus D3 lymph node dissection were analyzed retrospectively. The incidence of the chylous fistula in different surgical methods( the Laparotomy Group and the laparoscopy group ) and different surgical sites( left colon cancer and right colon cancer )was compared. Results Chylorrhea occurred in 22 patients( 3.5% ),including 2. 8%( 8/286 )in the Laparotomy Group and 4. 0%( 14/342 )in the Laparotomy Group,and the differences were no statistical significance( P >0.05 ). Incidence of the chylous fistula in radical resection of right -colon carcinoma plus D3 lymph node dissection was 7. 8%( 16/207 ),while incidence of the chylous fistula in radical resection of right - colon carcinoma plus D3 lymph node dissection was 1.4%( 6/421 ). There was statistical significance( P <0. 05 ), and there was no significant statistical significance between different TNM stages and incidence of the chylous fistula( P > 0. 05 ). Twenty - one patients received conservative treatment abrosia, total parenteral nutrition, somatostatin infusion, anti - infection, delaying removing drainage tubes ) were successfully cured,and one patient required re - operation. No patients had recurrence and died. Conclusion There is no statistical significance between the Laparotomy Group and the laparoscopy group. Radical resection of right - colon carcinoma plus D3 lymph node dissection is associated with higher risk for chyle leak. Conservative treatment is effective in early stage of chyle leak after radical resection of colon carcinoma plus D3 lymph node dissection.%目的总结结肠癌D3清扫术后腹腔乳糜漏的病因、治疗及预防经验.方法 对628例行结肠癌D3清扫术后发生乳糜漏病例进行回顾性分析,比较不同术式(开腹组与腹腔镜组)、不同部位(右半结肠与左半结肠)乳糜漏的发生率.结果 22例结肠癌患者(3.5%)术后发生乳糜漏,开腹组与腹腔镜组的发生率分别为2.8%(8/286)和4.0%(14/342),差异无统计学意义(P＞0.05);右半结肠D3清扫术后腹腔乳糜漏发生率为7.8%(16/207),左半结肠D3术式乳糜漏发生率为1.4%(6/421),两组相比,差异具有统计学意义(P＜0.05),乳糜漏的发生与肿瘤的TNM分期无明显关系.21例患者经保守治疗(治疗方法包括禁食、肠外营养、静脉使用生长抑素、抗感染治疗和延缓拔除腹腔引流管),均获治愈,1例行腹腔淋巴管漏口结扎术并局部使用医用胶治愈,无复发及死亡病例.结论 结肠癌D3清扫术开腹与腔镜在预防腹腔乳糜漏方面无明显差异,右半结肠D3术式是乳糜漏的高危因素,术后腹腔乳糜漏保守积极治疗有效.
  • 摘要：Objective To investigate the esophagus cancer mortality trend from 1985 to 2009 and project future ten years( 2010-2019 )in Henan province. Methods The data of esophagus cancer deaths and population data from 1985 to 2009 was drawn from Henan Provincial Center for Tumor and Vital Statistics Database. The mortality rates of periods and the age - standardized mortality rates by Chinese population( 1982 )and by world Segi's population were calculated. Joinpoint regression model was used to estimate the time trends of the mortality. Using the Leslie matrix to predict the population of Henan province during 2010-2019. The cancer mortality during 2010?019 in Henan province was projected by APC regression model. Results A total of 72 294 cases which died from esophagus cancer were reported by vital statistics and registry center in Henan province from 1985 to 2009. Among male, the age - standardized mortality rates by Chinese population was 49. 49 per 100,000 during period of 1985?989 year and decreased to 17. 72 per 100,000 during period of 2005?009 year. For female,the age - standardized rates decreased from 26.48 per 100,000 to 9.06 per 100,000. The estimated trend parameters by Joinpoint model showed that the standardized - mortality rate of esophagus cancer by Chinese standard population showed the decreasing trend in both male and female. There are two joinpoint in male and two joinpoint in female. Predicted model demonstrated that the average rate of population increased in Henan province will be 4. 38 %o during 2010-2019. The total population of the 15 cities and counties in Henan province in 2019 will reach 14. 87 million. In the future,the age - standardized mortality rates by Chinese standard population in male and female will be 17. 04 per 100,000 and 8. 73 per 100,000 during 2010-2014 ,and those in male and female are 16.67 per 100,000 and 7. 98 per 100,000 respectively during 2015-2019. Conclusion The mortality rates of esophagus cancer in Henan province showed the decreasing trend during the period of 2010-2019. But the mortality rate is still high. Prevention and control measures should be continued.%目的描述河南省1985-2009年间的食管癌死亡率变化趋势,预测2010-2019年食管癌死亡率.方法 从河南省癌症及生命统计中心数据库中抽取1985-2009年间死于食管癌的全部记录.计算各时期死亡率、中国人口标化死亡率(中标率)及世界人口标化死亡率(世标率),运用Joinpoint模型估计死亡率的时间变化趋势.采用莱斯利矩阵分性别预测2010-2019年人口数据;运用APC(Age-period-cohort)模型预测2010-2019年河南省居民食管癌死亡率.结果 1985-2009年间河南省生命统计登记中心报告死于食管癌总人数为72294人.男性中标率从1985-1989年间的49.49/10万下降到2005-2009年间的17.72/10万;女性相应时期的中标率由26.48/10万下降到9.06/10万.Joinpoint模型趋势参数显示:男、女性食管癌中标率呈逐年下降趋势,男性时间趋势有2个有意义的联结点,女性时间趋势有2个有意义的联结点.预测结果显示:2010-2019年河南省人口数平均增长率为4.38‰,2019年河南省15市县总人口数将达到1487万.2010-2014年的男性中标率为17.04/10万,女性中标率为8.73/10万.2015-2019年的男性中标率为16.67/10万,女性中标率为7.98/10万.结论 预测结果显示2010-2019年河南省居民食管癌死亡率呈下降趋势,但其死亡率仍较高,应继续实施相关预防控制措施.
  • 摘要：目的 探讨乳腺浸润性导管癌中HIF-1α、Twist1蛋白表达及其临床意义.方法 采用免疫组织化学SP法检测41例乳腺浸润性导管癌患者的癌组织与配对癌旁正常乳腺组织中HIF-1α与Twist1蛋白的表达情况.结果 癌组织中HIF-1α与Twist1的阳性表达率明显高于癌旁正常乳腺组织(HIF-1α:78.0% vs.19.5%,P＜0.001;Twist1:63.4% vs.12.2%,P＜0.001).在癌组织中,HIF-1α与Twist1的表达分别与TNM分期呈正相关(HIF-1α:r=0.322,P=0.04;Twist1:r=0.430,P=0.005),HIF-1α与Twist1蛋白的表达呈正相关(r=0.331,P=0.034).结论 乳腺浸润性导管癌中HIF-1α与Twist1蛋白呈高表达,且两者具有相关性,两者在乳腺浸润性导管癌的发生发展过程中可能起着重要的作用.%Objective The study investigated the expressions and significance of hypoxia - inducible factor - 1α( HIF-1α )and Twistl in invasive ductal carcinoma of breast( IDC ). Methods The expressions of HIF - 1α and Twistl proteins were detected by immunohistochemistry streptavidin perotridase( SP)method in breast carcinoma tissue and paired para - carcinoma tissues of 41 patients with IDC. Results The positive expression rates of HIF - 1α and Twist1 proteins in breast carcinoma tissues were significantly higher than in paired para - carcinoma tissues( HIF - 1α:78. 0% vs. 19. 5% ,P < 0. 001;Twistl:63. 4% vs. 12. 2% ,P < 0. 001 ). In breast carcinoma tissues,the expressions of HIF - la and Twistl in breast carcinoma tissues were both positively con-elated with the TNM stage( HIF - 1α:r = 0. 322 ,P = 0. 04 ; Twistl : r = 0. 43 , P = 0. 005 ), and the expression of HIF -1α was positively correlated with the expression of Twistl( ( r =0. 336 ,P =0. 019 ) )in breast carcinoma tissues. Conclusion The overexpressions of HIF -1α and Twistl were found in IDC and their expressions were positively correlated. They might play an important role in the development of IDC.
  • 摘要：目的探讨血管内皮生长因子(Vascular endothelial growth factor,VEGF)联合癌胚抗原(Carcino-embryonic antigen,CEA)检测应用于晚期非小细胞肺癌(Non-small-cell carcinoma,NSCLC)伴恶性胸腔积液患者的临床价值.方法应用电化学发光法和酶联免疫反应(Enzyme linked immunosorbent assay,ELISA)分别检测2010年1月-2011年12月湖北省公安县二人民医院收治的186例初治的晚期NSCLC伴恶性胸腔积液患者(研究组)的胸腔积液和血清的CEA和VEGF水平,与186例良性疾病并发胸腔积液患者(对照组)CEA和VEGF水平进行比较.且对研究组中的非鳞癌和鳞癌两个亚组的胸腔积液和血清的CEA和VEGF水平进行进一步比较.结果 研究组胸腔积液和血清中CEA、VEGF水平均高于对照组(P＜0.05),且研究组中非鳞癌亚组的胸腔积液和血清的CEA均显著高于鳞癌亚组,而VEGF则无统计学差异(P＞0.05);研究组血清中的VEGF联合CEA诊断的灵敏度和特异度分别为65.25%和61.99%,胸水中VEGF联合CEA诊断灵敏度和特异度分别为89.00%和79.33%.结论 联合检测胸腔积液和血清CEA、VEGF对晚期NSCLC伴胸腔积液患者具有重要的临床价值.%Objective To explore the clinical value of VEGF and CEA combination test in patients of late stage of non - small cell lung cancer with malignant pleural effusion. Methods To use ECLIA and ELISA method to test levels of CEA and VEGF in 186 cases of first line patients in the late stage of non - small cell lung cancer - study group - with malignant pleural effusion and compare them to that of the patients with benign pleural effusion. And non - squamous and squamous cell carcinoma in the study group in two subgroups of pleural effusion and serum CEA and VEGF levels were further comparison. Results We studied group pleural effusion and serum CEA. VEGF levels were higher than that of the control group( P < 0. 05 ). Non - squamous cell carcinoma subgroup pleural effusion and serum CEA were significantly higher in squamous cell carcinoma subgroup( P < 0.05 ),while there were no significant difference in two subgroups with VEGF( P > 0. 05 ). The sensitivity and specificity of combination test of VEGF and CEA in serum and pleural effusion were 65. 25% and 61. 99% , 89.00% and 79. 33% , respectively. Conclusion Combination test of CEA and VEGF in both pleural effusion and serum is of great clinical application value to patients in the late stage of non - small cell lung cancer with malignant pleural effusion.
  • 摘要：目的探讨恶性黑色素瘤术后十二指肠转移内镜像变化.方法对1例恶性黑色素瘤术后患者临床资料进行分析,通过内镜及病理检查明确诊断.结果 恶性黑色素瘤具有高转移性,内镜像与皮肤所见黑痣相似.结论内镜检查结合镜下病理检查,在诊断上消化道恶性黑色素瘤中起着关键作用.%Objective To investigate endoscope image of malignant melanoma's duodenum metastasis after operation. Methods We analysed the clinical date of a malignant melanoma patient after operation to clear diagnosis with endoscope and pathological examination. Results Malignant melanoma has strong metastasis and the endoscope image is similar to the mole of cutaeous. Conclusion Examination of endoscope combined with pathology plays an important role in diagnosis of upper digestive tract's malignant melanoma.
  • 摘要：At present, the attention on the application of tumor markers in tumors is growing. The tumor markers are wildly used in clinical detection of tumors, and have a certain value on the diagnosis and treatment of tumors. Studies found that CA125 is related to the development of systemic variety of malignancies, and can assist in the diagnosis of the disease, guide treatment and judge prognosis. So, it is one of the better tumor markers in clinical. In this article,we review the molecular structure of CA125 ,biological characteristics and its clinical significance in malignant tumor.%目的 目前,肿瘤标记物在肿瘤中的应用越来越受到重视,并被大量应用于临床检测,其对肿瘤的诊断和治疗具有一定的临床价值.研究发现,糖类抗原125(Cancer antigen125,CA125)与全身多种恶性肿瘤的发生、发展相关,可以协助疾病的诊断、指导治疗和判断预后,是临床上较理想的标记物之一.本文对CA125的分子结构、生物学特性及其在多种恶性肿瘤中的临床意义进行综述.
  • 摘要：目的研究高频彩色多普勒超声(高频彩超)在甲状腺癌和甲状腺腺瘤诊断和鉴别诊断中的临床应用价值.方法 对首都医科大学宣武医院2006年1月-2012年1月经手术病理证实的80例甲状腺癌和40例甲状腺腺瘤患者的高频彩超图像进行回顾性分析,比较二种疾病的声像特点、回声、钙化、血流特点等.结果 本组研究对象中80例甲状腺癌以乳头状癌和滤泡癌为主,高频彩超诊断准确率约为93.8%,其声像特点有:(1)甲状腺癌多数呈球形、类圆形或不规则形;(2)甲状腺癌边缘不规则,多数有毛刺、蟹足样、声晕发生率较低;(3)甲状腺癌多数以实质性回声为主;(4)肿瘤内部常伴有不同程度的钙化;(5)甲状腺癌血供大多较丰富,血供以高阻力为特点.但是部分甲状腺癌病灶较小,尤其是直径在0.5cm以下的病灶,容易漏诊.结论 高频超声在甲状腺癌和甲状腺腺瘤的诊断和鉴别诊断中有较大的临床实用价值,值得进一步推广并应用于其他肿瘤.%Objective To investigate the clinical value of high resolution ultrasound in the diagnosis and differential diagnosis of thyroid carcinoma. Methods A total 80 thyroid carcinoma and 40 thyroid adenoma patients were demonstrated by operation pathology between January 2006 and January 2012 which were included into this study. All these high resolution ultrasound images were analyzed restrospetively. The image characteristics, echo , calcification and blood flow of the two diseases were compared. Results The pathology of most thyroid carcinoma patients is papillary and follicular carcinoma. The correct rate of high resolution ultrasound is about 93. 8%. The characteristics of high resolution ultrasound of thyroid carcinoma included( 1 )the shapes of tumors were round and irregular,( 2 )the edge of tumors were irregular,most tumors with spurs, similar with crab foot,and rare halo,( 3 )most thyroid carcinoma were substantially hypoechogenic,( 4 )most thyroid carcinoma had calcification, ( 5 )most thyroid carcinoma had blood flow. However,the diameter of some thyroid carcinoma is small,especially lesions smaller than 0.5 centimeter, which is easily misdiagnosed. Conclusion The high resolution ultrasound showed high and clinical value in the diagnosis and differential diagnosis of thyroid carcinoma. This technology can be used in the diagnosis of other tumors,such as breast tumors.

    实用肿瘤学杂志的期刊信息

    • 曾用名：
    • 创刊时间：1987
    • 地区：CN
    • 语言：中文
    • 热门主题：黑龙江省肿瘤医院
    • 学科分类：

    实用肿瘤学杂志的获奖情况

    • • 黑龙江省第七届期刊优秀文章编辑暨第四届期刊装帧设计奖评选活动中荣获一等奖一项、二等奖两项

    实用肿瘤学杂志的收录情况

    • • 农业与生物科学研究中心文摘
    • • 化学文摘（网络版）
    • • 日本科学技术振兴机构数据库
    • • 中国科技核心期刊

    实用肿瘤学杂志的投稿信息

    • 投稿方式：在线投稿
    • 投稿须知：点击查看
    • 期刊官方网站：点击前往
    • 版面费：
    • 审稿费：元
    • 通讯地址：哈尔滨市南岗区保健路6号
    • 邮编：150081
    • 联系电话：0451-86298293 86623347
    • 邮箱：syzlxzz@126.com
    • 期刊栏目：综述；临床研究；个案；流行病学研究；基础研究；临床应用
    免费订阅本刊

    客服邮箱：kefu@zhangqiaokeyan.com

    京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有 出版物经营许可证

    • 客服微信

    • 服务号