奥沙利铂与7-羟基星形孢菌素对结肠癌HCT116细胞的杀伤作用及其机制的研究

摘要

Objective To observe the growth inhibitory effect of oxaliplatin( L - OHP )and( 7 - hydrox-ystaurosporine,UCN -01 )on HCT116 colon cancer cell line in single or combination treatment,and to investigate its mechanisms. Method The growth inhibitory effect was evaluated by MTT assay in single agent or combination treatment. Apoptosis was investigated by flow cytometry and Western blotting, and mitotic catastrophy was determined by HE staining. Results Combination treatment of L - OHP in low concentration and UCN - 01 has syn-ergistic effect on killing HCT116 colon cancer cell line,which was more effective than single treatment. AnnerxinV positive cell rate showed no difference between combination treatment and UCN - 01 alone. Cellular morphology of HCT116 cell was changed in combination treatment, and megakaryocytes and polykaryocytes in combination treatment were increased by approximately 10% compared with the single treatment. Conclusion Combinition treatment of L - OHP and UCN - 01 has synergistic inhibitory effect on HCT116 cell proliferation through inducing cell apoptosis and mitotic catastrophy.%目的 奥沙利铂与7-羟基星形孢菌素单独及联合应用,探讨诱导结肠癌细胞HCT116死亡的作用机制.方法应用MTT法检测 L-OHP、UCN-01单药及联用对HCT116细胞的增殖抑制作用;应用流式细胞术、Western blot方法检测药物处理后的结肠癌细胞凋亡情况;应用HE染色法检测丝裂灾变的形态改变.结果 低浓度L-OHP(6.25μmol/L)与UCN-01(100nmol/L)联用对结肠癌HCT116细胞的杀伤可产生协同作用,效果明显强于单独用药组;两药联用AnnexinV阳性细胞率与UCN-01单用无明显差别,但死亡细胞明显增加;两药联用HCT116细胞的形态发生改变,巨核多核细胞较单独用药组增多约10%.结论 L-OHP与UCN-01联用对HCT116细胞具有协同抑制作用,诱导HCT116细胞发生凋亡和丝裂灾变.