Objective In this study,Protosappanin A,Caesalpinia Sappan L extract and Cisplatin were combined with radiotherapy in gastric cancer cell SGC-7901 to investigate whether the Protosappanin A could in-crease radiosensitivity( SER) in gastric cancer SGC-7901 cells. This will be medication to create new areas of in-novation in the future. Methods The cell proliferation of SGC-7901 cells was detected by MTT assay. The rela-tionship between the effect of the Protosappanin A on cell proliferation and the time of action was determined. Caesalpinia Sappan L extract and Cisplatin were as controls. The fitted cell survival curve and clonal formation as-says were used to determine the SER to analyze the sensitizative effect of Protosappanin A. Results Protosappa-nin A could inhibit the growth of SGC-7901 cells,and its inhibitory effect is relatively weak. Its cytotoxicity has a time-and concentration-dependent manner. Cellular morphological changes were observed accompanying with increased concentrations and time treatments of Protosappanin A. Clonal formation experiment showed that the Protosappanin A significantly increased the radiosensitivity of SGC-7901 cells when compared to the radioactive group. They showed a statistically difference. Conclusion The inhibitory effect of the Protosappanin A on SGC-7901 cells in a concentration and time-dependent manner. Protosappanin A combined radiotherapy can improve the radiosensitization of cells,both of which may have synergistic anti-tumor effect.%目的 本研究将原苏木素A、苏木醇提物、顺铂分别与放疗联合作用于胃癌细胞SGC-7901细胞系,研究原苏木素A是否增加胃癌SGC-7901细胞系放射敏感性,为临床用药开创更新的领域.方法 体外培养人胃癌细胞SGC-7901细胞系,以苏木醇提物、顺铂为对照,MTT法检测原苏木素A对细胞增殖作用的影响,以及其与作用浓度和作用时间的关系;采用克隆形成实验,拟合细胞存活曲线,以单纯放疗组为对比,计算放射增比(SER),分析原苏木素A的增敏效果.结果 原苏木素A对人胃癌细胞SGC-7901有生长抑制作用,但抑制作用相对较弱,其细胞毒性有明显的时间和浓度依赖性.不同作用时间及作用浓度下的细胞形态发生明显的改变.克隆形成实验显示原苏木素A显著增加了SGC-7901细胞的放射敏感性,与单纯放射组对比,差异有统计学意义.结论 原苏木素A对胃癌SGC-7901细胞的抑制有浓度和时间的依赖性,联合放射治疗能够提高放射增敏作用,两者可能具有协同抗肿瘤作用.
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