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Effects of proliferation and apoptosis in human gastric cancer cell line SGC-7901 induced by Zedoary turmeric oil

机译:Zeedoary姜黄诱导的人胃癌细胞系SGC-7901中增殖和细胞凋亡的影响

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Objective To study the effect of Zedoary turmeric oil on proliferation and apoptosis of human gastric cancer cell line SGC-7901. The growth inhibition rate of different concentration of Zedoary turmeric oil on human gastric cancer cell line SGC-7901 were detected by trypan blue stain assay. Optical microscopy observation of cell morphological changes. DNA fragmentation detected by agarose gel electrophoresis. Flow cytometry was used to detect the changes of cell mitochondrial membrane potential, apoptosis rate and cell cycle distribution.Results: the optimal concentration wasl 10 μg/mL for 48h, and the IC50 values were 104.958 μg/mL. The typical DNA ladder on agarose gel electrophoresis, the apoptotic peak was showed by FCA detection. Conclusion: The Zedoary turmeric oil can induce apoptosis of gastric cancer cell SGC-7901.
机译:目的探讨Zeedoary姜黄油对人胃癌细胞系SGC-7901增殖和凋亡的影响。通过台盼蓝染色试验检测了人胃癌细胞系SGC-7901对Zeedoary姜黄油不同浓度的生长抑制率。光学显微镜观察细胞形态变化。通过琼脂糖凝胶电泳检测的DNA片段化。流式细胞术用于检测细胞线粒体膜电位,凋亡率和细胞周期分布的变化。结果:48小时的最佳浓度为10μg/ ml,IC 50值为104.958μg/ ml。琼脂糖凝胶电泳的典型DNA梯形,凋亡峰被FCA检测显示。结论:Zeedoary姜黄油可以诱导胃癌细胞SGC-7901的凋亡。

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