Objective To establish a method for determining catechin and epicatechin in Qili San . Methods HPLC was used for the determination. The chromatographic column was Prevail Select C18 (150 mm ×4. 6 mm, 5 μm) and the column temperature was 35℃. The mobile phase consisted of acetonitrile-water-phosphoric acid( 100 : 900:1) , The flow rate was 1 ml/min and monitored at 278 nm. Results The linear range of catechin was 30. 08-150.4 μg/ml, r = 0. 9999. The average recovery of catechin was 99.1% and RSD was 1.1%. The linear range of epicatechin was 12. 24-61. 20 μg/ml, r =0.9999. The average recovery of epicatechin was 97.4% and RSD was 1.2%. Conclusion This method is simple, time-saving and accurate. It can be used for routine analysis of catechin and epicatechin in Qili San .%目的 建立七厘散中儿茶素和表儿茶素的含量测定方法.方法 HPLC法,采用Prevail Select C18(150 mm ×4.6mm,5 μm)色谱柱,柱温:35℃；以乙腈-水-磷酸(100∶900∶1)为流动相；流速:1ml/min;检测波长:278 nm.结果 儿茶素、表儿茶素线性浓度范围分别为30.08 ～150.4 μg/ml,r=0.9999;12.24～61.20 μg/ml,r=0.9999;回收率分别为99.1％和97.4％,RSD分别为1.1％和1.2％.结论 本方法操作简便,测定结果准确可靠,可用于七厘散中儿茶素和表儿茶素的含量测定.
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