豆皮水溶性多糖组分SHP-3的物化性质研究

摘要

The water-soluble polysaccharide of soybean hull (SHP) was extracted by hot water and three fractions were obtained by using DEAE-cellulose anion exchange column.The physicochemical properties of the purified fractions SHP3, eluted with 0.3 M NaOH,were investigated.Gel permeation chromatography showed that the molecule weight of SHP3 was 45554.GC analysis of the monosaccharide composition showed that the molar ratio of Rah: Fuc: Ara: Xyl: Man: Gal : Glu was 3.55: 0.44:11.58: 1: 7.45: 5.12:1.12 and the content of galacturonic acid was 26.71％ ( wt.％ ).The ultraviolet spectra indicated that there was no presence of protein in SHP-3.The molar percentages of (1→2) glycosidic linkage, (1→4) glycosidic linkage and (1→6) glycosidic linkage were 11.4％ ,68.9％ and 19.7％ ,respectively.%对豆皮采用热水浸提得到豆皮水溶性多糖,通过DEAE-cellulose离子交换柱洗脱,得到3个组分.本文主要研究了采用0.3 M NaOH溶液洗脱纯化得到的组分SHP-3的物化性质.对SHP-3进行凝胶渗透色谱、气相色谱分析、紫外光谱及高碘酸氧化-Smith降解分析,结果表明:SHP-3的分子量为45554,其糖醛酸含量为26.71%(wt.%),单糖组成摩尔比为Rah∶Fuc∶Ara∶Xyl∶Man∶Gal∶Glu=3.55∶0.44∶11.58∶1∶7.45∶5.12∶1.12,紫外光谱在260～280 nm没有吸收峰,表明没有蛋白质或核酸等物质;高碘酸氧化-Smith降解结果表明SHP-3的连接结构以为(1→4)糖苷键为主,其摩尔比例占68.9%,(1→2)糖苷键占11.4%,(1→6)糖苷键占19.7%.