壳寡糖和壳聚糖对高脂膳食小鼠的降脂功能比较研究

摘要

本文比较研究了壳寡糖和壳聚糖对高脂膳食小鼠降脂作用的影响.选取雄性C57BL/6小鼠,随机分成4组:即Control组(正常日粮)、Model组(高脂日粮)、COS组(高脂+5％壳寡糖组)和CS组(高脂+5％壳聚糖组).饲喂17周,每周记录摄食量、称量小鼠体重.17周后进行小鼠血清、肝脏生化指标和RT-PCR分析.结果表明:壳寡糖和壳聚糖的添加可降低高脂膳食小鼠体重、血脂和肝脂水平,提高肝脂酶活性,缓解肝脏脂质过氧化水平,调节肝脏脂肪代谢基因,缓解脂质代谢紊乱.壳寡糖的降脂作用效果好于壳聚糖.%The hypolipidemic effect of chitosan oligosaccharide (COS) and chitosan (CS) was compared in mice fed high-fat diet.Male C57BL/6 mice were randomly assigned to four groups.Control group consumed a normal diet (Control).Model group was fed with a high-fat diet (Model).The COS mice were fed with high-fat diet supplement with 5％ COS (COS).The CS mice were fed with high-fat diet supplement with 5％ CS (CS).The diet intake and weight data were collected weekly,the biochemical indicators of serum and liver,the RT-PCR analysis in liver were determined at the end of the 17 week.The results indicated that supplementation of chitosan oligosaccharide and chitosan can reduce body weight,decrease the levels of serum lipid,liver lipid and increase the hepaticlipase capacity of HF mice.It can alleviate the liver lipid peroxidation,regulate the genes of liver fat metabolism and thus exert the hypolipidemic effect.In addition,chitosan oligosaccharide showed better hypolipidemic effect than chitosan.