大枣和葛根对酒精性肝病小鼠肝功能和肝组织 SIRT1表达的影响

摘要

Objective It is to explore the effect of Jujube and Pueraria on liver function and expression of SIRT1 in the rats with alcoholic liver disease.Methods 114 Kunming mice were randomly divided into control group (n=24) and model group (n=90), and the model group was given Red Star Erguotou 54 degrees 8 mL/(kg· d) by intragastric administration for 4 weeks.After that, 8 mice in each group were selected randomly and killed to detect serum ALT and SIRT1 expression and ob-serve the changes of liver pathology.The rest of the mice in model group were randomly divided into pathology group ( n=22), Jujube group (n=20), Pueraria group (n=20) and Bifendate group (n=20), all the mice were given Red Star Erg-uotou 54 degrees 8 mL/( kg· d) by intragastric administration every two days.On the basis above, pathology group, Jujube group, Pueraria group and Bifendate group were respectively given distilled water jujube extract, Pueraria root extract, Bifen-date at the dose of 0.01 mL/(kg· d).In 4 weeks, 8 weeks, 12 weeks after medication, serum ALT and SIRT1 expression were determined and the changes of liver pathology was observed in the mice in every group.Results Compared with the model group, the levels of serum AST, ALT were significantly lower while the expression of SIRT1 in liver tissue was higher with im-provements of liver function in Jujube grou and Pueraria group, the difference were statistically significant(P<0.05).Con-clusion Jujube and Pueraria both have a certain curative effect onalcoholic liver disease.%目的：探讨大枣和葛根对酒精性肝病小鼠肝功能和肝组织SIRT1表达的影响。方法114只昆明种小鼠随机分为对照组24只、模型组90只，模型组给予54度红星二锅头8 mL／（ kg· d）灌胃4周后，2组各随机取8只杀死取材，检测小鼠血清转氨酶和肝脏SIRT1蛋白表达水平，并观察肝脏病理改变情况；模型组剩余82只小鼠再次随机分为病理组22只、大枣组20只、葛根组20只和联苯双酯组20只，均继续隔日灌胃54度红星二锅头8 mL／（ kg· d）。在此基础上，病理组给予蒸馏水0．01 mL／（ kg· d）灌胃，大枣组给予大枣提取物0．01 mL／（kg· d）灌胃，葛根组给予葛根提取物0．01 mL／（kg· d）灌胃，联苯双酯组给予联苯双酯0．01 mL／（ kg· d）灌胃，观察灌胃4周、8周、12周小鼠血清转氨酶、肝脏病理切片和肝脏SIRT1蛋白表达水平。结果与病理组比较，大枣组、葛根组血清AST、ALT水平均下降，肝组织病理改善，肝组织SIRT1蛋白表达水平升高，差异均有统计学意义（P均＜0．05）。结论大枣和葛根均对酒精性肝病有一定疗效。