目的：观察含蓝莓花色苷的谷物发酵物（简称蓝禾，LH）对小鼠的抗疲劳作用。方法雄性昆明小鼠80只，分2批进行实验。（1）第1批实验：昆明小鼠随机分为对照组和低、中、高剂量LH干预3组，每组10只小鼠，其中对照组小鼠灌胃蒸馏水17．5 ml／kg体质量，LH干预组小鼠灌胃剂量分别为8．75、17．5、35．08 ml／kg体质量。连续灌胃28 d，测定小鼠负重游泳时间。（2）第2批实验：昆明小鼠随机分为对照组及高剂量LH干预组，每组20只小鼠，分别灌胃蒸馏水和LH，灌胃剂量均为35．08 ml／kg 体质量。连续灌胃28 d，测定小鼠血乳酸（ LA）、血清尿素氮（BUN）和肝糖原的含量。结果与结论（1）与对照组比较，LH干预组小鼠负重游泳时间均显著延长（ P＜0．01），高剂量LH干预效果最好；（2）LH干预组小鼠游泳后20 min，LA含量呈下降趋势（P＞0．05）、BUN含量明显降低（P＜0．05）、肝糖原储备量显著增高（P＜0．05）。 LH具有显著的抗疲劳作用。%Objective To investigate the anti-fatigue activity of fermented grain-containing blueberry anthocyanins ( LANHE,LH) in mice.Methods Experiments were conducted in two phases .In the first phase , forty mice were randomly divided into four groups:control group(distilled water,dw) and three LH administration groups (8.75,17.5 and 35.08 ml/kg body mass).In the second phase, mice were randomly divided into two groups:high-dose LH group (35.08 ml/kg body mass) and control group (dw 35.08 ml/kg body mass).After four weeks, a forced swimming test was performed and the biochemical parameters related to fatigue were examined .Results and Conclusion The administration groups showed a significant increase of swimming time to exhaustion compared with the control group , especially the high-dose group ( P<0.001).There was no significant change in the blood lactate between the two groups , but at 20 min after swimming, the lactic acid(LA) contents of the high-dose group were lower than in the control group (P>0.05).LH could significantly increase the liver glycogen contents and decrease the serum contents (P<0.05).These data indicate that LH has anti-fatigue activity and can elevate the exercise tolerance in mice .
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