反相高效液相色谱法检测鱼肉中的ATP关联物

摘要

The method for determination of ATP-related compounds in fish was established. The content of ATP-related compounds in fish were determined by RP-HPLC with a Sepax Bio-Cls（250mm × 4.6mm ID, 5 lam, 200A.）column, and UV detector at a wave length of 254 nm（BW 16 nm）. Mobile phase was 60 mmol/L KH2PO4 and 60 mmol/L K2I-IPO4 （pH=6.68） and flow rate was 0.6mL/min. The separation was achieved in 20 min. All the calibration curves were linear in the mass concentration range of 1 - 400 mg/L. The average recoveries of the standard additions were between 98.3% - 104.1%, and the LOD were between 15 -40μg/L for ATP-related compounds. This method proved to be reliable and reprodcible.%建立同时测定鱼肉组织中6种ATP关联物的高效液相色谱方法。采用反相高效液相色谱分离模式,使用Sepax Bio-C18（250mm×4.6mm,5μm,200A。）色谱柱,以60mmol/L磷酸二氢钾、60mmol/L磷酸氢二钾组成的缓冲液（pH6.68）为流动相等比洗脱,流速0.6mL/min;紫外检测波长为254nm,带宽为16nm。6种组分在20min内基线分离,平均加标回收率在98.3%～104.1%之间,线性范围为1～400mg/L,检出限在15～40μg/L之间。该方法简便、准确,易于移植。