采用传统微生物分离手段从中高温大曲中分离纯化得到黄曲霉菌株NJSYS-15。通过菌落观察、镜检以及分子生物学手段得知其为黄曲霉属；利用AFPA培养基验证其产毒能力，采用酶联免疫法对其产黄曲霉毒素B。进行定量检测，结果表明，其在液态发酵进行到9d时，黄曲霉毒素B1达到最高值13．6ug／kg。在进行固态模拟发酵时，黄曲霉菌株的接种量加大至10‰，黄曲霉毒素B1含量为3．2ug/kg，仍在国家标准限制以内。%A. flavus strain NJSYS-15 was isolated from medium-high-temperature Daqu through traditional microbial isolation method. It was identified as Aspergillus flavus by microscopic observation on the colony and molecular biology methods. Its toxin-producing capacity was verified by AFPA culture medium and the quantity of the produced afiatoxin B1 was detected by Enzyme-linked immunosorbent assays (ELISA). The results showed that aflatoxin B1 reached to the maximum of 13.6 ug/kg after 9 d liquid fermentation. However, during simulated solid fermentation in the Lab, even as the inoculating quantity of NJSYS-15 increased to 10 ‰, the content of the produced aftatoxin B1 was only 3.2 ug/kg, still within the limits of national standards.
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