Objective To observe the inhibitory effect of Pseudomonas aeruginosa against staphylococci to provide a new clue for the drug research and development in treating Staphylococci infection .Methods Forty-nine clinically isolated no-repeat strains of Pseudomonas aeruginosa were collected and their inhibitory effect against Staphylococcus aureus was observed by using the agar-well diffusion method ,cross-streaking method and co-culture method .Whether having correlation between endotoxin and its inhibi-tion against Staphylococcus was investigated by detecting the endotoxin level in different concentrations of Pseudomonas aerugino -sa .Results The strains PA2224 and PA2288 with strong inhibitory effect and PA2503 and PA2142 strains without inhibitory effect were screened by using the agar-well diffusion method .The cross streak method and co-incubation experiment suggested that the metabolites of Pseudomonas aeruginosa could suppress the growth of Staphylococci .The bacterial solution level of Pseudomonas aeruginosa was positively correlated with endotoxin .There was no correlation between the endotoxin level and the inhibitory effect of Pseudomonas aeruginosa .Conclusion Partial Pseudomonas aeruginosa have stronger inhibitory effect upon staphylococcus ,their metabolites have a chance to be made into bactericides and be applied in clinic ,which may have very good application value in Staph-ylococci infection .Endotoxin has no relation with inhibitory effect .%目的 观察铜绿假单胞菌对葡萄球菌的抑制作用,为治疗葡萄球菌感染的药物研发提供新线索.方法 收集临床分离出来的49株不重复铜绿假单胞菌,通过琼脂打孔法、十字交叉划线法和共孵育试验检测铜绿假单胞菌对葡萄球菌的抑制作用;通过检测不同浓度铜绿假单胞菌内毒素水平,研究内毒素与其抑制葡萄球菌生长是否存在相关性.结果 通过琼脂打孔法筛选出抑制作用强的PA2224菌株和PA2288菌株,无抑制作用的PA2503菌株和PA2142菌株.十字交叉划线法和共孵育试验提示铜绿假单胞菌代谢产物可抑制葡萄球菌生长.铜绿假单胞菌菌液浓度与内毒素水平呈正相关.内毒素水平高低与铜绿假单胞菌抑制作用无相关性.结论 部分铜绿假单胞菌对葡萄球菌属有较强抑制作用,其代谢产物有机会被制成杀菌药物应用于临床中,对临床上葡萄球菌属感染可能有很好的应用价值.内毒素与抑制作用无相关性.
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