脑胶质瘤组织中 Beclin-1的表达及与预后的关系

摘要

目的：探讨人脑胶质瘤组织中自噬相关基因Beclin-1的表达及与预后的关系。方法：采用免疫组化SP法及RT-PCR技术检测43例脑胶质瘤组织（包括Ⅰ级5例，Ⅱ级17例，Ⅲ级12例，Ⅳ级9例）及11例非瘤脑组织中Beclin-1蛋白和mRNA的表达，同时对随访资料进行Kaplan-Meier生存分析。结果：非瘤脑组织，Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级脑胶质瘤组织中Beclin-1蛋白和mRNA的表达水平差异均有统计学意义（ F＝99．962和41．951，P均＜0．001）。Ⅲ级、Ⅳ级脑胶质瘤组织中Beclin-1蛋白和mRNA的表达水平低于Ⅰ级、Ⅱ级脑胶质瘤组织和非瘤脑组织（P＜0．05），Ⅰ级、Ⅱ级脑胶质瘤组织中Beclin-1蛋白的表达水平低于非瘤脑组织（P＜0．05）。43例患者随访到35例，以脑胶质瘤组织中Beclin-1蛋白表达水平的中位数为界限，将35例患者分成Beclin-1蛋白低表达组（16例）及高表达组（19例），两组的Kaplan-Meier生存曲线差异有统计学意义（χ2＝4．962，P＝0．026），Beclin-1蛋白高表达组预后优于低表达组。结论：Beclin-1可作为判断人脑胶质瘤预后的新指标用于临床。%To explore the relationship between the prognosis and the expression of autophagy -related gene Bec-lin-1 in human glioma tissue .Methods:Immunohistochemistry and RT-PCR technology were used to detect the expressions of Beclin-1 protein and mRNA in 43 cases of glioma tissue ( including 5 cases of grade Ⅰ,17 cases of grade Ⅱ,12 cases of grade Ⅲand 9 cases of grade Ⅳ) and 11 cases of non-tumor brain tissue .The follow-up data were analyzed by Kaplan-Meier survival analysis .Results:There were significant differences in the expressions of Beclin-1 protein and mRNA a-mong non-tumor brain tissue, gradeⅠ,Ⅱ,ⅢandⅣglioma tissue(F=99.962 and 41.951,P<0.001).The expressions of Beclin-1 protein and mRNA in grade Ⅲand grade Ⅳ glioma tissue were lower than those in grade Ⅰ,grade Ⅱglioma tissue and non-tumor brain tissue(P<0.05).The expression of Beclin-1 protein in gradeⅠand gradeⅡglioma tissue was lower than that in non-tumor brain tissue(P<0.05).A total of 35 out of the 43 cases were followed up, 16 cases with low expression of Beclin-1 protein and 19 with high expression based on the median expression level .The Kaplan-Meier survival curves of the 2 groups differed from each other (χ2 =4.962,P=0.026), and the prognosis of high expression of Beclin-1 protein group was better than that of the low expression group .Conclusion:Beclin-1 may be a new indicator to predict the prognosis of human glioma .