桑黄液体深层发酵培养基优化研究

摘要

The mycelial biomass and polysaccharide concentrations were used as main indicators to explore the optimal culture medium of submerged fermentation of Phellinus linteus by means of orthogonal tests ,with nitrogen sources and carbon sources determined through single-factor tests .The orthogonal results showed that the optimal carbon and nitrogen sources were of sucrose and NH4Cl respectively .And the optimal culture medium were of sucrose 70 g/L ,NaCl 1 g/L ,NH4Cl 10 g/L ,KH2PO40 .5 g/L ,MgSO40 .5 g/L ,with pH natural and the maximal mycelial biomass fermented up to 2 .814 g .Under the conditions of 5% inoculation quantity ,50 mL culture volume and fermented temperature at 28 ℃ ,the mycelial biomass reached maximal at 120 h ,then tended to decline ,and the pH value of fermented broth decreased with the fermentation process and then remained practically stable after 120 h .%以菌丝体生物量和多糖产量为主要指标,对桑黄(鲍氏层孔菌)的液体深层发酵培养基进行了优化,通过单因素试验筛选碳源、氮源,通过正交试验优化桑黄液体深层发酵的培养基.结果表明:蔗糖为最佳碳源,氯化铵为最佳氮源;最适培养基各成分的质量浓度为蔗糖70 g/L、氯化铵10 g/L、磷酸二氢钾0.5 g/L、硫酸镁0.5g/L、氯化钠1g/L,pH值自然,以此条件发酵最大菌丝量为2.814g.在接种量5%、装液量50mL、28℃发酵过程中发现,发酵120 h时桑黄菌丝体生成量最大,随后呈下降趋势;发酵液p H值随着发酵进程呈现明显下降,在120 h后基本趋于稳定.