超声波辅助提取茶叶中黄酮物质的条件研究

摘要

Ethanol aqueous solution was used to extract total flavonoids from tea under ultrasonic assistance. To optimize extraction yield of total flavonoids, response surface methodology ( RSM ) was employed to perform it. The optional values of variables were as follows: ultrasonic treatment time 35. 30 min, solid/liquid ratio 1: 15. 40, ethanol concentration in aqueous solution 60. 6％, ultrasonic power 557. 83 W, the total flavonoid yield obtained under such conditions was 8. 513 mg/mL , which was extremely consistent with the experimental value with a relative error of 0. 6％.%为确定茶叶中总黄酮超声波提取的最佳工艺条件,应用Box-Behnken设计方法,以黄酮提取率为指标,采用响应曲面法对主要工艺参数进行优化并得到回归模型.结果表明:提取时间为35.30 min,料液比为1∶15.40,乙醇浓度为60.6%,超声波功率为557.83 W时,茶叶中黄酮提取率为8.513 mg/mL;回归模型的预测值与实际值的相对误差为0.6%,该回归方程与实际情况拟合较好.