EGCG 对细胞的促氧化作用研究进展

摘要

EGCG, which is the most abundant catechin in tea, has been demonstrated to possess a wide range of biochemical and pharmacological activities. Recent investigations have revealed that EGCG functions as a pro-oxidant. In this review, we discussed the pro-oxidative mechanism of EGCG in cell culture. Auto-oxidation of EGCG, influenced by the media types, dose- and time-dependent of EGCG addition, contents of serum and pH value etc., forms environmental oxidative stress in cell medium. Furthermore, EGCG produce intracellular ROS and mtROS, and trigger the Fenton reaction to create OH- indirectly. In particular, pro-oxidation of EGCG regulate intracellular transcription factors, signalling pathways, and cell surface receptor to exert many biological actions in cell.%EGCG 是茶叶中主要的儿茶素类物质，具有多种生物活性。近年来，EGCG 促氧化作用得到了广泛关注。本文对国内外细胞实验中 EGCG 的促氧化作用进行综述。EGCG 在细胞培养基中发生自动氧化可以形成细胞外氧化应激环境，这种自动氧化受到培养基种类、EGCG 浓度与处理时间、血清含量及 pH 值等因素影响。在细胞内，EGCG 直接提高细胞内活性氧（Reactive Oxidative Species, ROS）和线粒体 ROS，或者通过Fenton 反应间接产生 OH-。EGCG 可以影响细胞内转录因子、信号通路与表面生长因子受体的表达与传递，从而影响细胞的一系列生命活动。