Objective To detect the serum levels of melanocyte antibodies and explore the relation between melanoma antigen recognized by T-cells (MART-1) and vitiligo in children. Methods The serum samples were collected from children with vitiligo to test the autoantibodies, and divided into low- and high-titer group according to the test results. Melanocytes were incubated with the serum samples, and the changes of melanocyte surface antigen were evaluated using specific MART-1 antibody. Results The serum melanocyte antibody levels in children with vitiligo were significantly higher than those in normal subjects. The expression level of melanocyte surface antigen MART-1 increased obviously after incubation of the melanocyte with high antibody titer serum samples, and MART-1 was found to specifically bind to specific MART-1 antibody. Conclusion Melanocytes MART-1 may correlate to the autoimmune mechanism in children with vitiligo.%目的 了解儿童白癜风患者血清中抗黑素细胞抗体情况及探讨黑素瘤抗原-1(MART-1)与儿童白癜风的关系.方法 采集患儿血清.检测自身抗体,根据抗体滴度高低分成两组,与正常人黑素细胞进行孵育,利用MART-1特异性抗体检测孵育后黑素细胞表面抗原MART-1的变化.结果 白癜风患儿血清抗黑素细胞抗体滴度升高.与高滴度抗体患儿血清孵育后的MART-1表达明显增加,并能与MART-1特异性抗体丰H结合.结论 部分儿童白癜风是免疫相关的,MART-1与儿童白癜风可能相关联.
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