乙型肝炎病毒聚合酶与UXT蛋白的潜在相互作用鉴定

摘要

目的 研究乙肝病毒聚合酶(HBVPol)在肝细胞内潜在的相互作用的蛋白质因子,为揭示HBvPol发挥功能的分子机制,进一步了解HBV在细胞内复制的机制提供依据.方法 构建重组诱饵质粒pGBKT7-Pol,利用GAL4酵母双杂交系统筛选人肝cDNA文库得到与HBV Pol相互作用的蛋白质因子,再通过GSST-Pull down验证蛋白间的相互作用.结果 酵母双杂交筛选得到宿主蛋白Ubiquitously Expressed Transcript(UXT),GST-pull down确证蛋白间的相互作用.结论 UXT为一潜在的与HBV Pol相互作用的肝细胞蛋白质因子,这可能为研究HBV PoI在病毒生活周期中的功能提供线索.%Objective To investigate the function of hepatitis B virus polymerase (HBV Pol) in the viral life cycle by screening the proteins interacting with HBV polymerase. Methods The HBV Pol gene was constructed into the pGBKT7 vector. GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol. GST-pull down assay was applied to confirm the protein interactions. Results Ubiquitously expressed transcript (UXT) was selected by the yeast two-hybrid system. GST-pull down assay confirmed the in vitro interaction between HBV Pol and UXT. Conclusion UXT is a potential interactor of HBV Pol, and this protein interaction may provide clues of the function of HBV Pol in HBV life cycle.