视黄醇对人脐带间充质干细胞表皮生长因子、干细胞因子、集落刺激因子1和白血病抑制因子的影响

摘要

目的 研究视黄醇(维生素A)对人脐带间充质干细胞表达表皮生长因子(EGF)、干细胞因子(SCF)、集落刺激因子l(CSF1)和白血病抑制因子(LIF)的影响.方法 分离鉴定人脐带间充质干细胞,待细胞生长稳定后分为4组,分别用含12％FBS、12％ FBS+1 μmol/L视黄醇、15％血清替代品(KSR)和15％ KSR+1 μmol/L视黄醇的DMEM/F12培养液培养,3d后收集细胞及培养液上清,用RT-PCR和ELISA分析视黄醇对人脐带间充质干细胞生成细胞因子EGF、SCF、CSF1和LIF的影响.结果 分离所得细胞具备人脐带间充质干细胞免疫表型特征,细胞因子EGF、SCF和CSF1在mRNA和蛋白质水平均表达,而LIF只在mRNA水平表达;添加视黄醇(1μmol/L)后,SCF和CSF1在mRNA、蛋白质水平均显著增加(P＜0.05),而EGF和LIF变化不显著(P≥0.05).结论 视黄醇(l mol/L)可促进体外培养的人脐带间充质干细胞生成细胞因子SCF和CSF1.%Objective To investigate effects of retinol on the expressions of epidermal growth factor (EGF),stem cell factor (SCF),colony-stimulating factor 1 (CSF1) and leukemia inhibitory factor (LIF) in cultured human umbilical-derived mesenchymal stem cells (UCMSCs).Methods Human UCMSCs were isolated from human umbilical cord and identified for immunophenotypes.The cells were then cultured in DMEM/F12 media supplemented with 12％ fetal bovine serum (FBS),12％ FBS+1 μmol/L retinol,15％ knockout serum replacement (KSR) and 15％ KSR+ 1 μmol/L retinol.The expressions of the cytokines EGF,SCF,CSF1 and LIF in the cells were detected using RT-PCR and ELISA.Results The isolated cells exhibited characteristic immunophenotypes of human UCvSCs and expressed EGF,CSF1 and SCF at both mRNA and protein levels but not LIF protein.Retinol (1 μmol/L) significantly promoted the expressions of SCF and CSF1 at both mRNA and protein levels but did not result in changes of EGF and LIF expressions in human UCMSCs.Conclusion Retinol at the concentration of 1 μmol/L can promote expression of SCF and CSF1 in human UCMSCs in vitro.