芥菜型油菜黄化突变体L638-y的遗传及黄化基因gr1的分子标记

摘要

【目的】研究芥菜型油菜黄化突变体L638-y黄化性状的遗传规律,并初步定位黄化基因gr1。【方法】以芥菜型油菜黄化突变体L638-y分别与正常绿色的渭源大黄芥、2598进行杂交,根据杂交F1、F2和BC1材料单株的叶色调查结果进行遗传分析;以从L638-y与2598杂交后代选育得到的近等基因系（Near-isogenic line,NIL）为材料,选用SRAP、SSR、RAPD和AFLP分子标记,采用BSA（Bulk segregant analysis）法,初步定位黄化基因gr1。【结果】芥菜型油菜黄化突变体L638-y的黄化性状受2对隐性核基因控制,将其分别命名为gr1、gr2。将黄化基因gr1初步定位在2个AFLP分子标记EA4TG4和EA7MC1之间,其与2个标记间的遗传距离分别为33.6与21.5cM。【结论】芥菜型油菜黄化突变体L638-y的黄化性状受2对隐性核基因控制,且黄化基因gr1初步定位在EA4TG4和EA7MC1之间。%【Objective】 Inheritance of chlorophyll-deficient mutant L638-y in Brassica juncea and preliminary mapping of chlorophyll-deficient gene gr1 were done in this study.【Method】 The chlorophyll-deficient mutant L638-y was hybridized with the two normal lines,Weiyuandahuangjie and 2598,respectively.The resulting F1,F2 and BC1 populations were used to investigate the inheritance of chlorosis by checking leaf color phenotype in these progenies.The NIL(Near-isogenic line)derived from the progeny of the cross between L638-y and 2598 was used to map one of the chlorophyll-deficient genes-gr1 using SRAP,SSR,RAPD and AFLP with BSA(Bulk segregant analysis)method.【Result】 The chlorosis of L638-y was controlled by two pairs of recessive nuclear genes,namely gr1 and gr2.The chlorophyll-deficient gene gr1 was preliminary mapped between molecular markers EA4TG4 and EA7MC1,and the genetic distances to the markers were 33.6 cM and 21.5 cM,respectively.【Conclusion】 The chlorosis of L638-y was controlled by two pairs of recessive nuclear genes,and the chlorophyll-deficient gene gr1 was preliminary mapped between molecular markers EA4TG4 and EA7MC1.